application of immunoprecipitation

1. 2019/12/31 Co-Immunoprecipitation (Co-IP) - Profacgen  (https://www.profacgen.com) Home (/) Services Products APP & TECH Contact Us About Us (https://www.profacgen.com/contact- (https://www.profacgen.com/about- us.htm) us.htm) Co-Immunoprecipitation (Co-IP) Home (http://www.profacgen.com) / Services / Protein Analysis (https://www.profacgen.com/protein-analysis.htm) / Protein Interaction Analysis Services (https://www.profacgen.com/protein-  Sign In (https://www.profacgen.com/SignIn.aspx) / Register interaction-analysis-services.htm) / Co-Immunoprecipitation (Co-IP) (https://www.profacgen.com/SignUp.aspx) https://www.profacgen.com/Co-Immunoprecipitation-Co-IP.htm 1/5

2. 2019/12/31 Co-Immunoprecipitation (Co-IP) - Profacgen Co-Immunoprecipitation (Co-IP) Co-IP is a classic technology widely used for protein-protein interaction identification and validation. Based on the specific immunological interaction between the bait protein and its antibody, co-IP has become an effective and reliable method in detecting the physiological interaction between proteins. The principle of the co-IP technique is as follows: Many intracellular protein-protein interactions are retained when cells are lysed under non-denaturing conditions. The bait protein, for example, protein X, can be captured by its specific antibody stabilized to agarose beads. If there is another protein, the prey protein, for example, protein Y, binds to protein X in vivo, the protein X – protein Y complex can then be precipitated together by the antibody. Subsequently, through the investigation of protein Y, we can confirm the protein X – protein Y interaction, or discover new interactors of protein X. Principle of co-Immunoprecipitation https://www.profacgen.com/Co-Immunoprecipitation-Co-IP.htm 2/5

3. 2019/12/31 Co-Immunoprecipitation (Co-IP) - Profacgen The advantage of this technology includes: Both the bait and prey proteins are in their native conformation in the co-IP assay. The interaction between the bait and prey proteins happens in vivo with little to no external influence. The limitation of this technology lies in: Low affinity or transient interaction between proteins may not be detected. The result of Co-IP could not determine whether the interaction is direct or indirect, since the possibility of involvement of additional proteins could not be ruled out. The result of co-IP reflects the physiological protein interactions in vivo. If researchers have predicted potential interactors, co-IP can be coupled with Western Blot assay as a way of protein interaction validation. If no prediction is made, co-IP can be coupled with Mass Spectrometry to identify new interactors. Our Service Advantages: Customized service for each clients’ requirements Highly reproducible experimental results Short turn-around time Best price in the market https://www.profacgen.com/Co-Immunoprecipitation-Co-IP.htm 3/5

4. 2019/12/31 Co-Immunoprecipitation (Co-IP) - Profacgen Please contact us for more details of our professional Co-IP service. (https://www.profacgen.com/wp-content/themes/profacgen/pdf/Co- Immunoprecipitation (Co-IP) Service-Service Introduction.pdf) See the procedure of co-IP service and how you should submit your sample (protein sample/cell sample/tissue sample…). Our experts will design your co-IP experiment according to your specific requirements and we will provide detail data of the result and procedure. Click here to download the Service Instruction. (https://www.profacgen.com/wp- content/themes/profacgen/pdf/Co-Immunoprecipitation (Co-IP) Service-Service Introduction.pdf) See more details on the principle and protocol of co-IP on our website. We also provide troubleshooting guides for the problems you may account in your experiment. See more… (/Co-immunoprecipitation-Co-IP-Technical.htm) SERVICES  Protein Production (https://www.profacgen.com/protein-production.htm)   Bioinformatics Platform Introduction  (https://www.profacgen.com/Bioinformatics-Platform-Introduction.htm)  Protein Analysis (https://www.profacgen.com/protein-analysis.htm)  ChIRP-Seq Service (https://www.profacgen.com/services/protein-analysis/chi… * Complement Assays (https://www.profacgen.com/services/protein-analysis/c…  * Enzyme Activity Assay Service (https://www.profacgen.com/enzyme-activity-…  * Nucleic Acid-Protein Interactions (https://www.profacgen.com/services/protei…  * Protein Aggregation Analysis (https://www.profacgen.com/protein-aggregati… * Protein Interaction Analysis Services (https://www.profacgen.com/protein-int…  * Bio-Layer Interferometry (BLI) (https://www.profacgen.com/bio-layer-interferom… * Co-Immunoprecipitation (Co-IP) (https://www.profacgen.com/Co-Immunoprecip… * https://www.profacgen.com/Co-Immunoprecipitation-Co-IP.htm 4/5

5. 2019/12/31 Co-Immunoprecipitation (Co-IP) - Profacgen Isothermal Titration Calorimetry (https://www.profacgen.com/isothermal-titratio… * Membrane Protein Binding Screening and Profiling (https://www.profacgen.com/… * Protein Binding Site Mapping (https://www.profacgen.com/protein-binding-site-… * Proximity-dependent Biotin Identification (BioID) Service (https://www.profacgen… * Pull Down Assay (https://www.profacgen.com/pull-down-assay.htm) * Surface Plasmon Resonance (SPR) Service (https://www.profacgen.com/surface-… * Yeast Two-Hybrid Screening (https://www.profacgen.com/yeast-two-hybrid-scre…  * Screening and Profiling Services (https://www.profacgen.com/screening-and-…  * Transcription Factor Analysis (https://www.profacgen.com/transcription-fact… *  Drug Development (https://www.profacgen.com/drug-development.htm)  INQUIRY (https://www.profacgen.com/order.htm?i1=Co- Immunoprecipitation (Co-IP)) CONTACT US  Email: info@profacgen.com  Tel: 1-631-448-8149  Fax: 1-631-938-8127  45-1 Ramsey Road, Shirley, NY 11967, USA Copyright ©2010 - 2019 Profacgen. All rights reserved. | Terms and Conditions (https://www.profacgen.com/term-and- conditions.htm) | Privacy Policy (https://www.profacgen.com/privacy-policy.htm) https://www.profacgen.com/Co-Immunoprecipitation-Co-IP.htm 5/5