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Post-translational modification (PTM) refers to the covalent, usually enzymatic modification of proteins, and protein process during or after protein biosynthesis. Protein post-translational modification (PTM) increases the functional diversity of the proteome by the modifying proteins with functional groups, such as phosphate, acetate, amide groups, or methyl groups, and influences almost all the aspects of normal cell biology and pathogenesis. It plays a key role in many cellular processes such as cellular differentiation, protein degradation, signaling and regulatory processes, regulation of gene expression, and protein-protein interactions. The modifications genererally include phosphorylation, glycosylation, ubiquitination, nitrosylation, methylation, acetylation, lipidation and proteolysis and influence almost all aspects of normal cell biology and pathogenesis. Therefore, characterization of PTM, including the modification categories and modified sites, is critical in the study of cell biology and disease diagnostics and prevention.
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Post-translational Modification Analysis Post-translational enzymatic modification of protein biosynthesis. Protein the functional diversity of the functional groups, such as phosphate, groups, and influences almost all the aspects pathogenesis. It plays a key role in many cellular differentiation, protein degradation, signaling and regulation of gene expression, and protein-protein modifications genererally include phosphorylation, ubiquitination, nitrosylation, methylation, acetylation, proteolysis and influence almost all aspects of normal cell pathogenesis. Therefore, characterization of PTM, including the categories and modified sites, is critical in the study of cell disease diagnostics and prevention. modification (PTM) refers to the covalent, usually proteins, and protein process during or after post-translational modification (PTM) increases proteome by the modifying proteins with acetate, amide groups, or methyl of normal cell biology and processes such as cellular regulatory processes, interactions. The glycosylation, lipidation and biology and modification biology and Identification It can be post-translational enrichment steps are stability of also critical an advanced post-translational of Post-Translational Modifications (PTM) is a tedious process. affected by many factors. For example, most of the modifications are present in very low level. Therefore, necessary before identification process. Additionally, modification, and detection efficiency of mass spectrometry are factors in PTM identification process. Creative Proteomics offers analytical platform for the characterization of various modifications (PTM). overview Function of PTMs: Category of PTMs Functions Phosphorylation activity Ubiquitination Glycosylation Acetylation Lipidation Affect the activity and subcellular location of a protein MethylationAffect protein comformation and its affinity to other proteins Proteolysis Remove peptide sequences or regulatory domains Change protein comformation; Activate/inactivate catalytic Target protein for degradation Direct a protein to its destination Affect protein comformation and its affinity to other proteins Types of PTMs analysis service we provide:
Phosphorylation Glycosylation Ubiquitination S-Nitrosylation Biotinylation Methylation N-Acetylation Lipidation S-myristoylation S-prenylation Alkylation Glutamylation Workflow of our PTMs analysis service: Digestion of proteins into small fragments Protein separation and analysis using LC/MS/MS Database search PTM mapping Full protein annotation Technology platform: 2D Fluorescence Difference Gel Electrophoresis (DIGE) Liquid Chromatography (LC) High Performance Liquid Chromatography Matrix Assisted Laser Desorption Ordering Procedure: (HPLC) Ionization Mass Spectrometry (MALDI-MS) Ordering Procedure Our customer service representatives are available 24 hours a day, from Monday to Sunday.
