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Cell Migration Assay Kit* 96-well, 2-D Assay for Investigating Cell Migration of Adherent Cell Lines * Patent Pending. Platypus Technologies, LLC 5520 Nobel Drive, Suite 100 Madison, WI 53711 Toll Free: (866) 296-4455 Phone: (608) 237-1270 Fax: (608) 237-1271 TECHSUPPORT@PLatypustech.com.
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Cell Migration Assay Kit* 96-well, 2-D Assay for Investigating Cell Migration of Adherent Cell Lines * Patent Pending Platypus Technologies, LLC 5520 Nobel Drive, Suite 100 Madison, WI 53711 Toll Free: (866) 296-4455 Phone: (608) 237-1270 Fax: (608) 237-1271 TECHSUPPORT@PLatypustech.com
Assay Contains: Oris™ Cell Migration Assay Inserts in 96-well Plate Opaque Mask Inserts in 96-well Plate Opaque Mask Permits Easy-to-Read Results Insert Removal Tool Insert Removal Tool Easy-to-Read Results Easy-to-Read Results Page 2
Observe Migrated Cells Using Microscope or Plate Reader Oris™ Cell Migration Assay Procedure Four Simple Steps1. Apply the Mask2. Add Cells & Incubate3. Remove Inserts & Incubate4. Observe Migrated Cells Add Cells to the Well Incubate to Allow Cells to Seed Into Outer Annular Region of Well Remove Insert Apply the Mask Incubate to Allow Cells to Migrate Into Central Analytic Region of Well Seeded Cells that HAVE NOT Migrated into the Central Analytic Region are Blocked from View Page 3
Results of Oris™ Cell Migration Assay HT 1080 Cell Migration/Proliferation HT 1080 Cell Migration Pre-Migration (T= 0) Page 4 Post-Migration (T= 21 hrs)
Advantages of the Oris™Cell Migration Assay • High Reproducibility • Quantitative Kinetic and EndpointMeasurements • 2 Dimensional Closure Assay • Easy to Use / Less Hands-On-Time • No Specialized Equipment • Permits Limited Morphological Examination During • Migration • Amenable to High-throughput Analysis • May use Multiple Probes Simultaneously Page 5
Contact information for the Oris™ Cell Migration Assay: Platypus Technologies 5520 Nobel Drive, #100 Madison, WI 53711 Phone: 608-237-1270, x 303 Email: rgupta@platypustech.com Web: www.platypustech.com Page 6