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nuclear mitotic apparatus protein 1

Nuclear matrix proteins (NMPs) make up the internal structural framework of the nucleus and are associated with such functions as DNA replication, RNA synthesis, and hormone receptor binding.

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nuclear mitotic apparatus protein 1

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  1. numa1 numa1 Official Full Name Official Full Name nuclear mitotic apparatus protein 1 Background Background Nuclear matrix proteins (NMPs) make up the internal structural framework of the nucleus and are associated with such functions as DNA replication, RNA synthesis, and hormone receptor binding. Work by Nakayasu and Berezney, Zeitlin et al., and Berrios et al. has indicated that NMPs are involved in regulation and coordination of gene expression. The identification of cell-type specific nuclear matrix proteins supports their potential contribution to cellular differentiation and tissue development. Although the nuclear matrix has been shown to be highly insoluble in vitro, it is now known that cell death releases soluble nuclear matrix proteins that can be detected in culture supernatant and other fluids containing dead and dying cells. The antibodies employed in the Cell Death Detection (Nuclear Matrix Protein) ELISA were developed to nuclear matrix proteins isolated from tissue culture cells by standard procedures. Both antibodies have been shown to react with lambda gt11 cDNA clones carrying published sequence from nuclear mitotic apparatus protein (NuMA), a nuclear matrix protein. More specifically, the Capture Antibody for NMP 41/7 reacts with a lambda gt11 cDNA clone encoding a peptide of approximately 60 kDa, located in the amino terminus of the published sequence for NuMA, and the FITC-anti-NMP 41/7 detector antibody reacts with a lambda gt11 cDNA clone encoding a peptide of ~96 kDa, located in the carboxy- terminus of the published sequence for NuMA. Because the level of NMP 41/7 detected in culture supernatant is a function of the number of dead and dying cells, the assay may be useful to quantify cell death. Other methods used to measure cell death include MTT, 51CR, trypan blue staining and ATP level. In comparison to these methods, the NMP 41/7 assay is advantageous since it measures antigen released directly from dying cells, therefore allowing differentiation between cytostatic and cytotoxic effects. Also, the NMP 41/7 assay requires only a small aliquot of material for testing, and does not require manual cell counts for quantification. In addition to measuring cell death in tissue culture, NMP 41/7 has been shown to be a useful marker for quantitative determination of engraftment, extension of disease and treatment response of human leukemia in SCID mice. The level of NMP 41/7 detected in SCID mouse serum correlates with human-derived tumor mass because the Cell Death Detection (Nuclear Matrix Protein) ELISA

  2. antibodies detect only human nuclear matrix protein. The antibodies have been shown to be reactive with a number of human cell lines; they may not react with all human cells. The antibodies have not been shown to be reactive with non-human cells. The user should make an initial determination of the assay's suitability for a particular use. Synonyms Synonyms NUMA; SP-H antigen; centrophilin stabilizes mitotic spindle in mitotic cells; structural nuclear protein Recombinant Recombinant Proteins Proteins  Cell & Tissue Lysates Cell & Tissue Lysates Cat.# Cat.# Product name Product name Source (Host) Source (Host) Species Species Tag Tag Price Price NUMA1-1409H Recombinant Human NUMA1, His-tagged E. coli Human His +Inquiry NUMA1-1544H Recombinant Human NUMA1 Protein, His-tagged E. coli Human His +Inquiry NUMA1-28047TH Recombinant Human NUMA1 protein, GST-tagged Wheat Germ Human GST +Inquiry NUMA1-4109C Recombinant Chicken NUMA1 Mammalian Cells Chicken His +Inquiry Involved Pathway NUMA1 involved in several pathways and played different roles in them. We selected most pathways NUMA1 participated on our site, such as Caspase cascade in apoptosis, Cell Cycle, Cell Cycle, Mitotic, which may be useful for your reference. Also, other proteins which involved in the same pathway with NUMA1 were listed below. Creative BioMart supplied nearly all the proteins listed, you can search them on our site. Pathway Name Pathway Name Pathway Related Protein Pathway Related Protein

  3. Caspase cascade in apoptosis DIABLO; CFL2; NUMA1; CRADD; ACTA1; LMNB1; SATB1; DFFA; CASP2; PARP1 Cell Cycle GADD45AB; RAD21A; SETB; CDKN2C; RAD50; CSNK2A1; CLASP1; CEP290; GMNN; LIN52 Cell Cycle, Mitotic DCTN1; BORA; CENPP; TUBGCP3; LIN52; MAPRE1A; CEP63; OFD1; CDK11A; NUMA1 Centrosome maturation CDK11B; ODF2; TUBGCP3; MAPRE1A; CEP72; ODF2B; SDCCAG8; CETN2; NUMA1; TSGA14 G2/M Transition ODF2B; CEP152; TUBGCP3; CDK11A; CEP290; DCTN1A; CEP78; CEP57; ACTR1A; LIN37 Mitotic G2-G2/M phases CDK11B; TCTN2; TUBGCP2; CDK11A; LIN9; TUBG2; TUBGCP4; BORA; FOXM1; NUMA1 Recruitment of NuMA to mitotic centrosomes TUBG2; TUBGCP5; TUBGCP4; TUBGCP3; TUBGCP2; NUMA1 Recruitment of mitotic centrosome proteins and complexes TUBG2; TUBGCP4; TUBGCP5; CDK11A; NUMA1; CDK11B; TUBGCP3; TUBGCP2 Protein Function NUMA1 has several biochemical functions, for example, microtubule binding, protein binding, structural molecule activity. Some of the functions are cooperated with other proteins, some of the functions could acted by NUMA1 itself. We selected most functions NUMA1 had, and list some proteins which have the same functions with NUMA1. You can find most of the proteins on our site. Function Function Related Protein Related Protein Function Function microtubule binding Related Protein Related Protein KIAA1128; MAP1S; CHD4A; MAP1B; VPS41; MID2; CDK5RAP2; LC3; FNTA; MAP1LC3B Function Function protein binding Related Protein Related Protein RFX4; POLB; SOX14; CC2D1B; HIST3H3; BBS5; TMEM86B; DNM1L; GLYAT; MAB21L3 Function Function structural molecule activity Related Protein Related Protein KRT94; LOR; TUBG2; IVL; KRT85; KLHL3; CYT1L; LELP1; FGG; CYT1 Interacting Protein

  4. NUMA1 has direct interactions with proteins and molecules. Those interactions were detected by several methods such as yeast two hybrid, co-IP, pull-down and so on. We selected proteins and molecules interacted with NUMA1 here. Most of them are supplied by our site. Hope this information will be useful for your research of NUMA1. GNAI1; GPSM2; CCDC57; FLJ13057; TERF2IP; ESR1 NUMA1 Related Articles Catalano, A; O'Day, DH; et al. Rad53 homologue forkhead-associated kinase A (FhkA) and Ca2+-binding protein 4a (CBP4a) are nucleolar proteins that differentially redistribute during mitosis in Dictyostelium. CELL DIVISION 8:-(2013). Wiemels, JL; Bracci, PM; et al. Assessment of Autoantibodies to Meningioma in a Population-based Study. AMERICAN JOURNAL OF EPIDEMIOLOGY 177:75-83(2013).

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