targeted rna sequencing

1. Targeted RNA Sequencing Targeted RNA-sequencing selects and sequences subset transcripts of interests, increasing the coverage of a focused set of RNA sequences. It is an accurate method that generates genomic and gene-expression information of specific genome regions. The specific RNA sequences analysis enables RNA-Seq with high sensitivity and cost-effective access to NGS. By either targeted capture enrichment or amplicon- based approaches, Targeted RNA-Seq measures dozens to thousands of targets simultaneously. Enrichment assays also is a tool to identify both known and novel gene fusion partners in many sample types. Providing qualitative and quantitative information for differential expression analysis, allele-specific expression measurement, and gene fusion verification, targeted RNA-sequencing can help to understand tumor classification and progression, genetic diseases and RNA drug response. Cancer transcriptome sequencing provides valuable information about gene expression changes in tumors. Profiling RNA- based drug response biomarkers aids too uncover multiple drug- susceptible tumorigenic pathways and improve the efficiency and success rate of the drug development process. Advantages of targeted RNA Sequencing Provides a comprehensive analysis of the specific transcripts Choose validated pathway, cell, or disease-specific panels Compatible with low-quality, formalin-fixed, paraffin-embedded (FFPE) Fast turnaround time and Highest data quality Strand information on RNA transcripts Effective transcriptome and pathways analysis        Targeted RNA Sequencing workflow We offer integrated targeted RNA-Seq workflows that simplify the entire process, from library preparation to data analysis and biological interpretation. Sample Requirements

2. Recommended concentration should be 20ug/uL or more. A minimum of 2μg of the total is required. A260: A280 value should be between 1.8 and 2.0 RNA should be stored in nuclease-free water or RNA Stable. RNA must be validated using an assay such as a BioAnalyzer RNA chip. RIN value ≥ 7.0 Tissue, cell, blood, and blood products are acceptable.       Sequencing Illumina High throughput sequencer Flexible service options: single end or paired-end sequencing, optional reads number according to research goals.   Data analysis Raw data QC Alignment and TPM/RPKM/FPKM-based quantitation Expression analysis Statistics of SNPs/Indels Alternative splicing analysis GO and KEGG annotation       Source: https://www.cd-genomics.com/targeted-rna-sequencing.html