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Immunohistochemistry- IHC Introduction | Immunostaining

Immunohistochemistry is a powerful microscopy technique for visualizing cellular components for proteins. Immunhistostaining is a method to identify specific antigens within tissue sections utilizing a specific antigen antibody.

Immunostaining
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Immunohistochemistry- IHC Introduction | Immunostaining

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  1. The Basics of Immunohistochemistry Website: immunostaining.info

  2. Overview of Immunohistochemistry Immunohistochemistry (IHC) combines histological, immunological and biochemical techniques for the identification of specific tissue components by means of a specific antigen/antibody reaction tagged with a visible label. IHC makes it possible to visualize the distribution and localization of specific cellular components within a cell or tissue.

  3. Immunohistochemistry IHC is a method for detecting antigens or haptens in cells of tissue. can be used to locate particular cells and proteins Can be used to identify the cellular events.

  4. IHC protocol – basic steps Fixation: to keep everything in its place Antigen Retrieval : to increase availability of proteins doe detection Blocking: to minimize pesky background signals Antibody labeling and visulization– getting the pretty picture.

  5. Common method for protein detection ELISHA Weston Blot Enzyme assays Immunohistochemistry NMR

  6. General Antibody Struture

  7. Summary IHC – Immuno+ Histo+ Chemistry Detect antigens in cells of tissue Steps: Fixation Processing Cutting staining

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