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Immunohistochemistry Immunofluorescence Introduction to research

Immunohistochemistry Immunofluorescence Introduction to research. Why use it?. Look for specific protein expression within tissues Study development, diseases, and evolution. Western Blot. Same technology is used for western blotting. Protein expression: p63. How it works.

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Immunohistochemistry Immunofluorescence Introduction to research

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  1. ImmunohistochemistryImmunofluorescenceIntroduction to research

  2. Why use it? Look for specific protein expression within tissues Study development, diseases, and evolution

  3. Western Blot • Same technology is used for western blotting

  4. Protein expression: p63

  5. How it works

  6. Different methods – pro’s and con’s

  7. Double-Labeling Co-localization of proteins Compartmentalized expression Paracrine expression

  8. Immunofluorescence • Need special microscope for study

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