1 / 13

CLS1113 Introduction to Clinical Laboratory Practices

CLS1113 Introduction to Clinical Laboratory Practices. Reagent Test Strip : Blood, Protein, Leukocytes and Nitrites. Hematuria (Red Blood Cells). Intact RBCs present in the urine: severe exercise football, running, soccer, aerobics UTIs glomerulonephritis pyelonephritis

Jims
Download Presentation

CLS1113 Introduction to Clinical Laboratory Practices

An Image/Link below is provided (as is) to download presentation Download Policy: Content on the Website is provided to you AS IS for your information and personal use and may not be sold / licensed / shared on other websites without getting consent from its author. Content is provided to you AS IS for your information and personal use only. Download presentation by click this link. While downloading, if for some reason you are not able to download a presentation, the publisher may have deleted the file from their server. During download, if you can't get a presentation, the file might be deleted by the publisher.

E N D

Presentation Transcript

  1. CLS1113 Introduction to Clinical Laboratory Practices Reagent Test Strip: Blood, Protein, Leukocytes and Nitrites

  2. Hematuria (Red Blood Cells) • Intact RBCs present in the urine: • severe exercise • football, running, soccer, aerobics • UTIs • glomerulonephritis • pyelonephritis • kidney stones • tumors • menstruation

  3. Hemoglobinuria • Hemoglobin present: • hemolytic anemia • transfusion reaction • burns • malaria • Correlate with microscopic findings and clarity

  4. Myoglobinuria • Myoglobin: • heme-containing protein involved in the transport of oxygen in muscles. • muscle trauma • seizures • muscle diseases • electric shock • alcoholism (toxic effects of alcohol) • Exercise:  levels of Myoglobin

  5. Blood on the RTS • A positive test for blood can either be RBC’s, hemoglobin or myoglobin. Table 7-7. • False Negatives: protein, high S. G., increased nitrite

  6. Proteinuria • Usually one of three causes(Table 7-8) • excess production of proteins • damaged glomerulus • damaged renal tubules • There is a normal amount of urine protein. • Albumin, Globulins • Increased Proteins is often the FIRST indicator of renal disesase.

  7. Protein on the RTS “Protein Error of Indicators” Tetrabromphenol blue (Indicator dye) + Protein Chromogen releasesH+ ions (produces a color change) Most sensitive to albumin

  8. Protein on the RTS

  9. False positives: • high pH • cells • medications • Confirm with SSA • SSA will pick up other types of proteins also. • Microalbumin Test • used for diabetes monitoring

  10. Leukocyte Esterase (enzyme) • Enzyme produced by Polymorphonuclear Leukocytes (WBC) • WBCs usually indicate a UTI • Correlate with microscopic exam and nitrite

  11. Leukocyte Esterase (RTS) An Ester in the presence of an esterase will produce a color change. • False negatives: • increased glucose • protein • S.G.

  12. Nitrite • Many urinary tract pathogens convert nitrate to nitrite • Indicates presence of bacteria • The bacteria must remain in the bladder long enough for this to occur • Correlate with microscopic exam (3-4+) • Will not pick up all types of bacteria

  13. Nitrite Test Strip Nitrite + para-arsanilic acid produces a pink color change “Greiss Reaction”

More Related