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Bacterial Transformation

Bacterial Transformation. By Joyce Simko, Anita Beebe, Judy King and Sr. Clare Marie Klein. Overview: What is Bacterial Transformation?. The transformation of bacteria! The genetic information of a bacterial cell actually takes in new

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Bacterial Transformation

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  1. Bacterial Transformation By Joyce Simko, Anita Beebe, Judy King and Sr. Clare Marie Klein

  2. Overview:What is Bacterial Transformation? The transformation of bacteria! The genetic information of a bacterial cell actually takes in new genetic information and makes it a part of itself! It can then copy that sequence over and over and over and over and over and over…….. How? Why? Stay tuned!

  3. E. coli Escherichia coli is the most common bacterium in the human gut. It has been extensively studied in the laboratory and is an important research organism for molecular biology. E. coli reproduce very rapidly; a single microscopic cell can divide to form a visible colony with millions of cells overnight. Like all bacteria, E. coli has no nuclear envelope surrounding the bacterial chromosome and thus no true nucleus. All of the genes required for basic survival and reproduction are found in the single chromosome. http://www.phschool.com/science/biology_place/labbench/lab6/concepts1.html

  4. Plasmids • Plasmids are circular pieces of DNA that exist outside the main bacterial chromosome and carry their own genes for specialized functions. In genetic engineering, plasmids are one means used to introduce foreign genes into a bacterial cell. • Some plasmids have the ampR gene, which confers resistance to the antibiotic ampicillin. E. coli cells containing this plasmid, can survive and form colonies on LB agar that has been supplemented with ampicillin. Cells lacking the ampR plasmid are sensitive to the antibiotic, which kills them. An ampicillin-sensitive cell can be transformed to an ampicillin-resistant cell by its uptake of a foreign plasmid containing the ampR gene. The same can be said for the lac gene, which codes for lactose. If this gene is taken in, the organism can break down lactose. • To transform cells, you first need to make them competent to take up extracellular DNA. http://www.phschool.com/science/biology_place/labbench/lab6/concepts1.html

  5. In the Beginning… • God said, “Let there be transformation!!!” • http://www.dnalc.org/ddnalc/resources/transformation2.html http://www.dnatube.com/thumb/2_202.jpg

  6. Transformation Procedure http://www.phschool.com/science/biology_place/labbench/lab6/concepts1.html

  7. Results http://www.phschool.com/science/biology_place/labbench/lab6/concepts1.html

  8. TEST TIME!!! • Place the Stages of Transformation in Order: C,B,D,E,A

  9. In the Classroom • STANDARDS RICH! • Unique & interesting ways to expand the scientific world of students • History of science (DNA Timeline) • Integrates Math, Science and History • Allows students to use many scientific instruments and processes • Explores new methods • Scientific inquiry—allows students to explore • Careers in science • Future of Science Research

  10. Lab Work Ohhhh, yea!!!

  11. DNA Timeline • http://www.dnai.org/timeline/index.html • kentsimmons.uwinnipeg.ca/ cm1504/mendel.htm

  12. S t r e t c h i n g Our Abilities! Working with high tech equipment: Modern Lab Mmmm, bonito!!!

  13. Modeling GREAT Science Teaching That's what I'm talkin about!

  14. Active Learners with our Colleagues Eureka!

  15. Opportunity to Share Curriculum Workin those brains!!!

  16. Grow in Confidence with new Computer Knowledge

  17. Best and Brightest Teachers

  18. Concluding Thoughts Thank you!!! The End!

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