RT-qPCR

1. Let's Create the Future of Life Science A More Cost-Effective Scientific Research Solution

2. About Us Trusted Brand Name with Cost Effective Products

3. About Us Beijing SBS Genetech Co.,Ltd. is one of the earliest Chinese companies providing custom oligonucleotides synthesis, gene synthesis and custom peptide synthesis. Since we founded in 2000, we have been committed to use technology to create a future that we are a proud of. Over decades, by providing safer, higher quality and more affordable products, we have served researchers in more than 40 countries, empowering them to create new fundamental knowledge in the field of biology.

4. About Us Today we not only provide custom oligos, gene synthesis and peptide synthesis services, we also offer the following products: DNA synthesis products, nucleic acids isolation & purification kits, DNA molecular weight markers, PCR-Related Products, RNA-Related Products, gene manipulation products, etc. With its remarkable characteristics, we have acquired a leading position in such fields, and contributed to life science development together with wonderful researchers. Thousands of papers have been published in the world's top academic journals with our products.

5. About Us From genome sequencing of E. coli to human beings, from basic molecular cloning technique to CRISPR-Cas-mediated genome editing, from earliest combinatorial synthesis of genetic networks to chemical synthesis of two S. cerevisiae chromosome arms, we have been witnessing the miracle created by life science throughout past decades. We are proud that we realized our dream and we believe the future will be brighter with our joint efforts.

6. Product Portfolio From Custom DNA Synthesis to CRISPR Gene Editing​

7. Custom DNA Synthesis DNA synthesis is a technology that links deoxynucleic acids (adenine, thymine, cytosine and guanine) together to form DNA. As the cornerstone of modern molecular biology, DNA synthesis plays a pivotal role in the field of synthetic biology. In addition to standard oligos synthesis, we also provide scientific research services such as long oligos synthesis, phosphorothioate oligos (S-Oligo) synthesis, modified oligos synthesis, fluorescent oligos synthesis, and real-time quantitative PCR probes to meet your different needs.

8. Custom Peptide Synthesis Peptides are synthesized by condensation reaction of carboxyl group of one amino acid with amino group of another amino acid and are widely used in various fields such as antibody preparation, drug development and polypeptide vaccine development. Each of our polypeptides is accompanied by reliable HPLC and mass spectrometry data, detailed synthesis reports are provided, and the products are sent in a lyophilized state. Experienced staff can assist users in designing peptide chains and make appropriate recommendations for different needs of users, such as antibodies, special markers, large-scale synthesis, etc. In addition, we also offer Custom PNA Synthesis with high quality.

9. Custom Peptide Library A peptide library is a new technique for studying structure-function relationship for a protein. A peptide library contains a great number of peptides that have a systematic combination of amino acids. The peptide library provides a powerful tool for drug design, protein-protein interactions, and other biochemical as well as pharmaceutical applications. It also has wide applications in drug screening, target validation, epitope mapping, and vaccine development.

10. Gene Synthesis​ Gene synthesis is a technology that synthesizes genes by artificial methods, which is one of the means of gene acquisition. Compared with the acquisition of genes from existing organisms, gene synthesis does not need templates and is therefore not limited by the source of genes. We use unique gene synthesis design software, which includes a full set of tools to design ideal structural units, thus enabling rapid and efficient gene construction and synthesis in a single reaction. Please do not be limited by restriction sites and polylinkers, we will synthesize the various gene sequences you need.

11. Nucleic Acid Purification​ Nucleic acid purification is an important component of molecular biology and has a wide range of applications in medicine and biological sciences. Our nucleic acid purification kit uses first-class silica gel column technology (SiMax™ Spin Column, SiMax™ Genomic DNA Extraction, SiMax™ PCR Products/Agarose Gel Purification, SiMax™ Plasmid DNA Miniprep) and magnetic beads technology (VirusMag™ Magnetic Beads, VSep™ Magnetic Separators, VirusMag™ One-Step DNA/RNA Isolation Kit, VirusMag™ DNA/RNA Isolation Kit), which can purify DNA from various sources quickly and reliably. The purity of DNA purified by our kit is very high and it is suitable for many downstream applications such as sequence determination, cloning and cleavage.

12. PCR Related​ Polymerase chain reaction (PCR) is a widely used method in molecular biology, which can rapidly replicate millions to billions of specific DNA samples, enabling scientists to extract only a small amount of DNA samples for detailed research. We provide a variety of DNA polymerases (Taq, Bst, f-Pfu, sPfu) and corresponding PCR premixes, covering a wide range of scenarios such as high fidelity, high specificity, and rapid amplification. Scarlet™ series kits provide a perfect solution for blood direct PCR, and PrimeSNP™ Genotyping Kit uses Competitive Allele Specific PCR method for genotyping of purified DNA samples. High quality dNTPs (set, mix) are also supplied.

13. RNA Related​ Ribonucleic acid (RNA), as a key material for genetic information transmission and cell regulation, has been extensively studied in molecular biology. Like DNA, RNA is assembled in the form of nucleotide chains; but unlike DNA, RNA exists in nature in the form of single-stranded folds rather than paired double strands. We provide RNasin (RNase inhibitor), M-MLV reverse transcriptase and Carrier RNA to provide a complete raw material solution for RNA research. miAnalysis™ series are designed for microRNA quantitative research. And VirusMag™ series are designed for the isolation of viral RNA/DNA or bacterial DNA.

14. DNA Markers DNA markers are used to determine the approximate size of molecules on the gel during electrophoresis, based on the principle that the molecular weight is inversely proportional to the mobility through the gel matrix. We provide abundant DNA molecular weight standards that can be used for various fragment lengths. Our fragments of DNA markers have been purified separately by proprietary technology, so their quality is superior to industry standards.

15. Genetic Manipulation Gene manipulation is a process that uses biotechnology to manipulate genes directly to generate new DNA, and has been widely used in research, medicine, industrial biotechnology and agriculture. Our Premium™ Master Assembly Mix, Muta-direct™ Site-Directed Mutagenesis Kit and Topo Cloning kits (pBM23, pBM16A, pBM16K) provide efficient solutions for these types of demands.

16. Nucleic Acid Stain GoodView™ is a safer nucleic acid stain, an alternative to the traditional ethidium bromide (EB) stain for detecting nucleic acid in agarose gels. It emits green fluorescence when bound to DNA or RNA. This new stain has two fluorescence excitation maxima when bound to nucleic acid, one centered at 268 nm and another at 294 nm. In addition, it has one visible excitation at 491 nm. The Fluorescence emission of GoodView bound to DNA is centered at 530 nm. Our GoodView™ Nucleic Acid Stain is also included on New Products, Science Magazine, January 11, 2019.

17. Biochemicals​ Biochemistry, as its name implies, is a discipline that studies chemical processes in organisms, often referred to as biochemistry. It is mainly used to study the structure and function of various intracellular components, such as proteins, sugars, lipids, nucleic acids and other biomacromolecules. We provide variety types of high quality biochemical reagents to meet these demands, including Proteinase K, Mutant Proteinase K, Besta™ LE Agarose, IPTG, X-Gal, DNase I (RNase Free), Enterokinase, SUMO Protease, TEV Protease, PreScission Protease (PSP), Bovine Serum Albumin, T4 DNA Ligase, etc.

18. Nuclease A nuclease is an enzyme capable of cleaving the phosphodiester bonds between nucleotides of nucleic acids. Nucleases variously effect single and double stranded breaks in their target molecules. Here we offer various high quality nucleases, including RNase A, RNase H, Thermostable RNase H, Benzonase Nuclease, Uracil N-Glycosylase, T7 Endonuclease I, T5 Exonuclease, Shrimp Alkaline Phosphatase, Terminal Transferase, etc. Nuclease 3’ 5’ 3’ 5’ 5’ 5’ 3’ 3’ 3’ 3’ 5’ 5’

19. Lab Supplies​ We provide various high quality common lab supplies, including ELISA Plates, Cell Culture Dishes, Cell Culture Plates, Microcentrifuge Tube, Centrifuge Bottles, Centrifuge Tubes, PCR Plates, PCR Tubes, Pipette Tips, Serological Pipettes, 96-well Deep-Well Plate, Conical Cryovials, SiMax™ Spin Column, with high cost-effective ratio to make your experiments smoother!

20. CRISPR Gene Editing​ CRISPR gene editing is a genetic engineering technology in molecular biology that is used to modify the genome of an organism. By introducing Cas9 nuclease into cells with a guide RNA (gRNA) complex, specific locations of the cell genome will be cleaved so that existing genes can be removed and/or new genes added in vivo. We provide high quality chemically modified Synthetic sgRNAs to provide a more reliable solution for CRISPR gene editing.

21. Achievements SBS Genetech and scientists contribute to the development of life science together

22. Examples: Harvard University United States The mutants were constructed by PCR-based site-directed mutagenesis (Muta-directTM kit, Beijing SBS Genetech Co., Ltd., China). Imperial College London United Kingdom The wild CII263–272peptide (FKGE-QGPKGE), wild HA308–317peptide (YVKQNTLKLA), altered HA308–317peptide (YAKQATLALA), and irrelevant peptide (ALALTAQKAY) were synthesized by solid-phasetechniques (SBS Genetech). The purity was > 95%. National University of Singapore Singapore Ten peptides corresponding to the amino acids 20–39, 30–49, 42–61, 50–69, 60–79, 73–92, 88–108, 101–120, 111–130 and 124–144 of the Blo t 12.0101 protein were prepared by solid-phase synthesis with an automated peptide synthesizer, purified by high-performance liquid chromatography and (HPLC) and analysed by mass spectrometry(SBS Genetech, Beijing, China). Cornell University United States The PCR products were checked for effective amplification through electrophoresis in 1% agarose gels containing 1:20 GoldView (SBS Genetech Co., Beijing, China).

23. Examples: Tsinghua University China The locked nucleic acids (LNA) targeting ZIKV sfRNA were chemically synthesized and HPLC purified by Beijing SBS Genetech Co., Ltd. University of Michigan United States These oligonucleotides were designed to amplify a 250-bp segment of GAPDH that serves as an internal standard. The primers were purchased from SBS Genetech Co. Ltd, Beijing, China. Peking University China A Site‐Directed Mutagenesis Kit (SBS Genetech Co., Ltd) was then used to mutate the miR‐375 binding site in the 3′‐UTR of IGFBP3 and named as IGFBP3‐mutant‐type (MT) luciferase reporter plasmid. Johns Hopkins University United States Fluorescein isothiocyanate (FITC)‐labeled peptides (N‐terminal) usedthroughout this manuscript were synthesized from SBS Genetech(Beijing, China), and purified using reversed phase analytical high‐performance liquid chromatography (>99% purity).

24. Examples: The University of Hong Kong China The PCR reaction mixture consisted of 2 µL DNA (about 15 ng), 2.5 µL of 10 × PCR buffer, 1.5 µL of 25 mmol/L MgCl2, 1.5 µL of 2.5 mmol/L dNTPs, 1.5 U of Taq DNA polymerase (SBS Genetech Co., China), 2.0 µL each of 2.5 µmol/L IT1F and IT2R primers (synthesized by SBS Genetech Co., China) in a final volume of 25 µL. University of Toronto Canada Point mutations of RokC114-185 were generated using the site-directed mutagenesis kit (SBS Genetech). Kyoto University Japan Genomic DNA was extracted by Genome DNA Simax Kit (Beijing SBS Genetech Co., Ltd., China). Seoul National University South Korea Each 25 µL reaction mixture contained 50 ng DNA, 5 pmol of each primer, 2 µL PCR buffer [100mM Tris (pH 8.3), 500 mM KCl, 15 mM MgCl2], 250 µM of each dNTPs and 0.5-1 unit Taq DNA polymerase (SBS Genetech Co, Beijing, China).

25. Examples: University of Melbourne Australia After DNA extraction, extracts were loaded on 1% agarose gel in 0.5x Tris-Acetate-EDTA (TAE) buffer with GoodView Nucleic Acid Stain (SBS Genetech Co., Ltd., China) and were electrophoresed (100 V). University of Queensland Australia Reverse-transcription of total RNA into the first-strand cDNA was performed by an RT-PreMix kit (SBS Genetech) and a universal dT3AP [oligo(dT)-containing adaptor primer], which were directly used as templates for RACE (3 rapid amplification of cDNA ends) with primers MeuICK-F1 and 3AP (Figure 1A). University of Zurich Switzerland ISSR primers (SBS Genetech Co. Ltd, Beijing, China) were screened using the six samples of O. sinensis collected from the six spatially and clearly separated populations. University of Malaya Malaysia All the oligonucleotide probes and target were procured from SBS Genetech Co., Ltd (Beijing, PRC)

26. Thanks! Contact Us Room 11E, Building 2 No.2 Shangdi Information Road Haidian District Beijing 100085 China E-mail: tech@sbsbio.com Web: https://www.sbsgenetech.com/