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THE BENEFITS OF AUTOMATINGNGS LIBRARY PREP

Time and money may be saved, as well as errors avoided, by<br>using specialized automation solutions for preparing NGS<br>Library preparation, such as liquid handling equipment and<br>integrated microfluidic systems.

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THE BENEFITS OF AUTOMATINGNGS LIBRARY PREP

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  1. THE BENEFITS OF AUTOMATING NGS LIBRARY PREP https://tekmatic.com

  2. Increased use of next-generation sequencing (NGS) has made it a useful tool in the diagnostic journey of many patients. However, many labs are forced to depend on send-out services for NGS testing since the conversion of native DNA to a barcoded library detectable by NGS is a highly technical procedure that requires substantial time and chemicals and is prone to mistakes.

  3. Time and money may be saved, as well as errors avoided, by using specialized automation solutions for preparing NGSLibrary preparation, such as liquid handling equipment and integrated microfluidic systems. When all together, the advantages of automating NGS library creation in a clinical laboratory setting are substantial.

  4. When doing next-generation sequencing, what goes into making the library? There are two primary steps leading up to NGS sequencing, and both may be automated. First is sample preparation, which entails separating DNA from the collected tissue (e.g., blood, saliva) and subjecting it to chemical or mechanical disruption to release the DNA from the nucleus. The second phase, library preparation, entails a great deal of tedium and nuance. In order to get your library ready for short-read sequencing, you’ll need to do the following:-

  5. The first step is to fragment the DNA into manageable chunks that can be read by a sequencer. • Attach the DNA fragments to sample-specific adapters. • Attach the DNA-adapter hybrids to a solid state for amplification and sequencing • Time- and money-saver • The tedious and monotonous process of preparing NGS libraries is often regarded as the bottleneck of DNA sequencing. Clinical genetics labs waste time and money when they use a manual procedure since it involves more effort to prepare reagents is repetitious and is prone to mistakes.

  6. Thank You Address:- 7316 N Alpine Road Loves Park, IL 61111 Phone:- 815.282.1775 Website:- https://tekmatic.com

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