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Sun and Shade Acclimation of the Photosynthetic Apparatus in Gymnostomum aeruginosum

Sun and Shade Acclimation of the Photosynthetic Apparatus in Gymnostomum aeruginosum. Adam Yock 2003-04. http://www.scibridge.sdsu.edu/coursemats/introsci/diversity/chlorobionta/chlorobionta_cladogram.jpg. Chloroplast. Thylakoid. Chloroplast. Granum (stack of thylakoids).

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Sun and Shade Acclimation of the Photosynthetic Apparatus in Gymnostomum aeruginosum

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  1. Sun and Shade Acclimation of the Photosynthetic Apparatus in Gymnostomum aeruginosum Adam Yock 2003-04

  2. http://www.scibridge.sdsu.edu/coursemats/introsci/diversity/chlorobionta/chlorobionta_cladogram.jpghttp://www.scibridge.sdsu.edu/coursemats/introsci/diversity/chlorobionta/chlorobionta_cladogram.jpg

  3. Chloroplast

  4. Thylakoid Chloroplast Granum (stack of thylakoids)

  5. electron transport pathway Sun Photons ADP + P + NADP NADPH ATP LHC RC PSII PSI Thylakoid Chloroplast Granum (stack of thylakoids)

  6. Introduction • Reaction Centers (RC) • Beginning of electron transport chain • Contain chl a Light-Harvesting Complexes (LHC) • Photosystematic antennas • Contain chl a and b

  7. Reactions of Light-acclimated Specimens chl a/b LHC chl a/b chl a/b

  8. Previous Studies • Post and Vesk analyzed light adaptability in moss using chl a/b

  9. Purpose Analyze light adaptability in moss (Gymnostomum aeruginosum) using both a chlorophyll ratio (chl a/b) and a protein ratio (LHCp/RCp).

  10. Hypotheses • Peas: • Both chl a/b and LHCp/RCp would significantly change • Moss: • Neither chl a/b nor LHCp/RCp would significantly change

  11. 1: p = 6. 2464 x 10-5 p = 0.8868

  12. Table 1: Average Chlorophyll/gram of Fresh Weight

  13. Tris-Tricine 10-16% gradientElectrophoresis gel

  14. 2:

  15. 3:

  16. Table 2: Thylakoid Protein Ratios (LHCp/RCp) in Peas

  17. Table 3: Thylakoid Protein Ratios (LHCp/RCp) in Moss

  18. Further Study • Explore the apparent discrepancy between the change in chlorophyll ratios and the change in the protein ratios • Use at least three thylakoid isolations per specimen

  19. Acknowledgements • Dr. Verhoeven • Ms. Fruen • Breck Team Research 2003-04

  20. Sun and Shade Acclimation of the Photosynthetic Apparatus in Gymnostomum aeruginosum Adam Yock 2003-04

  21. Analysis Absorbance readings entered into the equations: • Chl a = 12.25 x A663.6 – 2.55 x A646.6 • Chl b = 20.31 x A646.6 – 4.91 x A663.6 • Chl a + Chl b = 17.76 x A646.6 + 7.34 x A663.6

  22. Discussion • Chlorophyll Ratios: • Pea chl a/b changed significantly (p = 6. 2464 x 10-5) • Agrees with hypothesis • Moss chl a/b did not change significantly (p = 0.8868 ) • Agrees with hypothesis

  23. Discussion • Protein Ratios: • Pea LHCp/RCp changed significantly (p = 1.44 x 10-5) • Agrees with hypothesis • Moss LHCp/RCp changed with marginal significance (p = 7.00 x 10-2) • Does not agree with hypothesis

  24. Methods: Collecting Moss/pea Samples Peas: • Grown from seed Moss: • Harvested from the banks of a small rivulet in St. Paul, MN

  25. Isolating Moss/pea Thylakoids • Prepared -Moss ground in liquid nitrogen -Peas blended in a blended • Vortexed and spun in centrifuge (moss twice, peas three times) • Aliquoted

  26. Measuring Moss Chlorophyll Content • Mixed with acetone -Moss ground in acetone -Peas diluted with acetone and vortexed • Spun in centrifuge (moss twice, peas once) • Read supernatant in spectrophotometer at 663.6 nm and 646.6 nm

  27. Moss/pea Sample Electrophoresis Preparation • Spun in centrifuge • Boiled

  28. Results

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