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J.M.Nguta a *, J.M.Mbaria a , D.W.Gakuya a , P.K.Gathumbi a , J.D.Kabasa b , S.G.Kiama a

Toxicity of Antimalarial Plant extracts from Kenyan biodiversity to the brine shrimp, Artemia salina L . (Artemiidae. J.M.Nguta a *, J.M.Mbaria a , D.W.Gakuya a , P.K.Gathumbi a , J.D.Kabasa b , S.G.Kiama a a University of Nairobi, Nairobi, Kenya b Makerere University, Kampala, Uganda.

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J.M.Nguta a *, J.M.Mbaria a , D.W.Gakuya a , P.K.Gathumbi a , J.D.Kabasa b , S.G.Kiama a

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  1. Toxicity of Antimalarial Plant extracts from Kenyan biodiversity to the brine shrimp, Artemia salina L. (Artemiidae J.M.Ngutaa*, J.M.Mbariaa, D.W.Gakuyaa, P.K.Gathumbia, J.D.Kabasab, S.G.Kiamaa aUniversity of Nairobi, Nairobi, Kenya bMakerere University, Kampala, Uganda

  2. Introduction • Malaria is the single most cause of ill health, death and poverty in sub-saharan Africa • There are as many as 300 m acute cases of malaria worldwide each year, resulting in 1m deaths • 90% of these deaths occur in sub-saharan Africa, and majority of victims are children aged less than 5 yrs • Malaria is a major obstacle to social economic growth in Africa, accounting for 40% of public health expenditure Nguta et al, 2010. Journal of Ethno pharmacology

  3. Introduction • In Kenya, 22M people are at risk, 70% of them are in rural areas • About 34,000 Kenyan children die every year from malaria compared to a total estimate of 42,000 people dead • 80% of people worldwide are estimated to use herbal remedies against common diseases including malaria. • However, few data are available on their safety.

  4. Introduction • The current study was designed to evaluate the acute toxicity of crude plant extracts used against malaria in Kenya in Artemia salina larvae

  5. Materials and methods Study site (Msambweni district)

  6. Materials and methods Preparation of extracts • Aqueous • Organic (CHCL3/MeOH, 1:1) –Cold maceration Acute toxicity determination • The procedure of Meyer et al (1982), was adopted for LC50 determination

  7. Results

  8. Results

  9. Discussion • Only 24% of the aqueous crude extracts used against malaria in Msambweni, Kenya are safe • Only 4.5% of organic extracts screened were found be safe in brine shrimp lethality assay • Majority of crude extracts could not make safe antimalarials • Further antimalarial and phytochemical work is underway

  10. Acknowledgements • Carnegie Corporation of New York through RISE-AFNNET (for funding) • RISE-AFNNET colleagues • ICOPHAI 2011 organizing committee for a travel grant and invitation • University of Nairobi (Granting paid leave) • ICOPHAI 2011 Participants (For their attention). AHSANTENI SANA!

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