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Overview of exosome strucrure

Overview of exosome strucrure. Components of the core protein of exosome complex. Archeal exosome. Csl4. Rrp4. Rrp41. Rrp42. Rrp45p. Archaeal exosome. S. solfataricus. Two RNase PH subunits: Rrp41 and Rrp42. Rrp42. Rrp41. 1. 248. 275. RNase PH. 1. RNase PH. 224. 187. 28. 19.

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Overview of exosome strucrure

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  1. Overview of exosome strucrure

  2. Components of the core protein of exosome complex Archeal exosome Csl4 Rrp4 Rrp41 Rrp42 Rrp45p

  3. Archaeal exosome S. solfataricus Two RNase PH subunits: Rrp41 and Rrp42 Rrp42 Rrp41 1 248 275 RNase PH 1 RNase PH 224 187 28 19 Two S1 homologs subunit : Rrp4 and Csl4 Rrp4 S1 KH 223 All-β 1 20 57 135 50 124 220 Cls4 S1 ZR All-β 179 1 9 145 47 122 165 58

  4. The archaeal exosome core is a hexameric ring structure with three catalytic subunits 41(mut):D182A 42(mut):E218A NATURE STRUCTURAL & MOLECULAR BIOLOGY VOLUME 12 NUMBER 7 JULY 2005

  5. Sequence alignment of Rrp41 and Rrp42 Pink : phosphate binding site

  6. View of the S. solfataricus Rrp41-Rrp42 Exosome Core Bound to a Short Single-Stranded RNA Molecular Cell, Vol. 20, 473–481, November 11, 2005 The RNA is bound with the 3’ end near the phosphate binding site and with the 5’ end toward the central channel.

  7. Detailed view of the structure at the RNA binding site

  8. RNA Binding Arginines Are Essential for Catalytic Activity Schematic of the interactions at the RNA binding site

  9. Structure of exosome bound A5, U8 and ADP polyA5 + Pi polyA4 + ADP

  10. Schematic of the reaction mechanism

  11. How does the RNA reach the active site?

  12. Archaeal exosome Two RNase PH subunits: Rrp41 and Rrp42 Rrp42 Rrp41 RNase PH RNase PH Two S1 homologs subunit : Rrp4 and Csl4 Cls4 Rrp4 S1 ZR All-β S1 KH All-β Archaeoglobus fulgidus Molecular Cell, Vol. 20, 461–471, November 11, 2005

  13. Structure of the nine subunit archaeal Rrp4- and Csl4- exosomes Archaeoglobus fulgidus [C] top view [A] top view [B] side view Molecular Cell, Vol. 20, 461–471, November 11, 2005

  14. Detailed view of the interface of the N-terminal domain of Rrp4 (red), Cls4 (red) and the C-terminal helix of Rrp41 (blue) Cls4-Rrp41 Rrp4-Rrp41

  15. Archaeal exosome V.S. bacterial PNPase PNPase RNase PH RNase PH Rrp42 Rrp4 (Csl4) Rrp41 Archaeal exosome RNase PH RNase PH KH-S1 Align RNase PH domain of Rrp41, Rrp42 and PNPase

  16. The structure of S. antibioticus PNPase with the phosphate analog tungstate bound to the at the PNPase catalytic sites PNPase RNase PH RNase PH Structure, Vol. 8, 1215–1226, November, 2000

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