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Surface Plasmon Resonance. Dayun Yu. Optical principle. Dispersion relation Advantage of the surface plasmon resonance. Surface plasmon wave (K sp ). Evanescent wave (K ev ). =. 2D-detector array. Condition of Resonance. Light (ω). :. Reflectivity. angle.
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Surface Plasmon Resonance Dayun Yu • Optical principle. • Dispersion relation • Advantage of the surface plasmon resonance
Surface plasmon wave (Ksp) Evanescent wave (Kev) = 2D-detector array Condition of Resonance Light (ω) : Reflectivity angle Theory of Surface Plasmon Resonance
w w0 0 K Dispersion Relation Theory of SPR Ksp= w/c x {(emr x es)/(emr + es)}1/2 Ks=w/c x (es)1/2 Kretschmann’s prism method Kev=Kp x sinq=w/c x (ep)1/2 x sinq Kev=Ksp (Resonance point : w0) qsp=sin x [(emx es)/{ep(emr + es)}]1/2 Fig. Dispersion of evanescent wave vector(kev) and surface plasmon wave vector(ksp)
Advantage of the SPR • Monitor binding rapidly • Measurement in real time • Process experiment without labels
Summery • Detection of binding is based on the SPR phenomenon • Advantage of SPR • Monitor binding rapidly • Measurement in real time • Process experiment without labels