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Positive ion TOF-SIMS images of a 50:30:20 d4-DPPC:POPC:d31-POPG LB film deposited on

Positive ion TOF-SIMS images of a 50:30:20 d4-DPPC:POPC:d31-POPG LB film deposited on gold –coated mica at p = 30 mN/m. Mass 50 corresponds to C 3 D 7 which is specific to d31-POPG, masses 104 and 184 correspond to choline and phosphocholine which are specific to POPC respectively,

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Positive ion TOF-SIMS images of a 50:30:20 d4-DPPC:POPC:d31-POPG LB film deposited on

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  1. Positive ion TOF-SIMS images of a 50:30:20 d4-DPPC:POPC:d31-POPG LB film deposited on gold –coated mica at p = 30 mN/m. Mass 50 corresponds to C3D7 which is specific to d31-POPG, masses 104 and 184 correspond to choline and phosphocholine which are specific to POPC respectively, masses 108 and 188 correspond to d4-choline and d4-phosphocholine specific to d4-DPPC and mass 739 corresponds to d4-DPPC-H+. These images show that POPC and d31-POPG are present in the LE phase while d4-DPPC is highly concentrated in the LC phase. Images are 50 x 50 mm2, 256 x 256 pixels with 1 shot/pixel. This set of images shows that TOF-SIMS can be used to determine the location of each phospholipid in an LB film of a mixture.  In this work the authors are trying to design a synthetic surfactant that will eventually replace animal extracted BLES which is currently administered to premature babies throughout Canada. Courtesy of Eleonora Keating, Univ. of Western Ontario

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