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Supplementary Figure 2. + CDK. - CDK. MBP-CDKF;1 (871T/A). MBP-CDKF;1 (166K/A). MBP-CDKF;1 (886T/A). MBP-CDKF;1 (166K/A). MBP-CDKF;1 (886T/A). MBP-CDKF;1 (106G/A). MBP-CDKF;1 (106G/A). MBP-CDKF;1 (871T/A). MBP-CDKF;1 (w). MBP-CDKF;1 (w). CDK only. kD. 1. 2. 3. 4. 5. 6. 7. 8.

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  1. Supplementary Figure 2 + CDK - CDK MBP-CDKF;1 (871T/A) MBP-CDKF;1 (166K/A) MBP-CDKF;1 (886T/A) MBP-CDKF;1 (166K/A) MBP-CDKF;1 (886T/A) MBP-CDKF;1 (106G/A) MBP-CDKF;1 (106G/A) MBP-CDKF;1 (871T/A) MBP-CDKF;1 (w) MBP-CDKF;1 (w) CDK only kD 1 2 3 4 5 6 7 8 9 10 11 Supplementary Figure 2. Kinase activities of mutant Ee;CDKF;1 proteins in vitro. Wild and mutant Ee;CDKF;1s were made as MBP-CDKF;1 fusion proteins (lanes 1-5, 7-11), and kinase activities were examined with the addition of its substrate CDK (+ CDK) or without the addition of CDK (- CDK). In lane 6, the reaction mixture contained no enzyme, only substrate CDK. MBP-CDKF;1 100 CDK 41 100 MBP-CDKF;1 CDK 41

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