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House Sparrow Nestlings as Sentinels for West Nile Virus in Saskatchewan. Caroline Millins , Alex Reid, Phil Curry, Mike Drebot , Peter Buck, Ted Leighton. Outline. Introduction Materials and Methods Results Discussion Conclusion. WNV Cycle.
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House Sparrow Nestlings as Sentinels for West Nile Virus in Saskatchewan Caroline Millins, Alex Reid, Phil Curry, Mike Drebot, Peter Buck, Ted Leighton
Outline • Introduction • Materials and Methods • Results • Discussion • Conclusion
Total number of cases of human WNV infections in Canada and SK
Five Hills Health Region
Dead Corvid WNV surveillance; • Provides an early warning of WNV activity, However • Dead Corvid submissions from rural areas are generally low.
Study Question Can House Sparrow nestlings be used as an alternate method of wild bird WNV surveillance in a rural, high risk area of Saskatchewan ?
2006 & 2007 2005
Methods N McTaggart Souris River 50km House Sparrow sample location Mosquito trap location
Diagnostic Testing West Nile virus RT-PCR VecTestTM
Results 2006 2007
House Sparrow eggs were collected in July from Weyburn and Estevan • WNV plaque reduction neutralisation carried out on yolks Measuring Immunity to WNV, 2007
Discussion 2006 • WNV activity appeared sharply localized in southeast SK from May 30 to August 5 2006 • Implications for rural surveillance
Discussion • House Sparrow nestling surveillance was not a sensitive marker of WNV activity 2007
Conclusions • House Sparrow nestling surveillance has not been a useful surveillance tool in southeast Saskatchewan • An alternate sentinel bird species could be considered for use in this area
Thankyou • Ted Leighton, CCWHC • Alex Reid, WCVM graduate • Phil Curry, Saskatchewan Health • Mike Drebot, PHAC • Peter Buck, PHAC • AnjuTumber, Prairie Diagnostic Services • Toni Hansen, Molecular Diagnostics, Regina • Patrick & Marnie Zimmer, CCWHC