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真核生物の転写と翻訳の動画 youtube から. http://www.youtube.com/watch?v=bk7PW1FKMTI&feature=player_embedded. FIGURE 2 | Promoter-proximal pausing and escape to productive elongation. From the following article: Breaking barriers to transcription elongation Abbie Saunders, Leighton J. Core & John T. Lis
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真核生物の転写と翻訳の動画 youtubeから http://www.youtube.com/watch?v=bk7PW1FKMTI&feature=player_embedded
FIGURE 2 | Promoter-proximal pausing and escape to productive elongation. From the following article: Breaking barriers to transcription elongation Abbie Saunders, Leighton J. Core & John T. Lis Nature Reviews Molecular Cell Biology 7, 557-567 (August 2006)
Polyclonal Antibodies http://www.gelifesciences.co.jp/newsletter/biodirect_mail/technical_tips/tips63.asp
Monoclonal Antibodies ミエローマ (http://users.rcn.com/jkimball.ma.ultranet/BiologyPages/M/Monoclonals.html) 脾臓 - - Screening the logic: Unfused myeloma cells cannot grow because they lack HGPRT. Unfused normal spleen cells cannot grow indefinitely because of their limited life span. However, Hybridoma cells (produced by successful fusions) are able to grow indefinitely because the spleen cell partner supplies HGPRT and the myeloma partner is immorta
Western Blotting 2nd Abの例 AP-labeled goat anti-rabbit IgG IR Dye 800-labeled goat anti-rabbit IgG (Figure 2) 1st Ab 2nd Ab http://www.motifolio.com/6111115.html
組換えタンパク質の Tag Tagによる検出 Tagによる精製 Tagによる相互作用解析
免疫沈降法 (immunoprecipitation:IP) http://www.act-nonpareil.com/products/pro2.html
PF0027と相互作用 解析するタンパク質と MSによる分子同定 (feat. Mr. Masuda) PF0099 LSU ribosomal protein L12A 50S ribosomal protein L31e 50S ribosomal protein L21e 50S ribosomal protein L14e 50S ribosomal protein L7Ae 30S ribosomal protein S6e 50S ribosomal protein L37e 30S ribosomal protein S8P 50S ribosomal protein L24e