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MICROBIAL GENETICS CONJUGATION (MATING) AND TRANSDUCTION

1. MICROBIAL GENETICS CONJUGATION (MATING) AND TRANSDUCTION (PHAGE-LIKE TRANSDUCING PARTICLES MOVE DNA). 2. MICROBIAL GENETICS - CONJUGATION. MALE, FERTILITY FACTOR, DONOR FEMALE, ABSENCE OF F FACTOR, RECIPIENT

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MICROBIAL GENETICS CONJUGATION (MATING) AND TRANSDUCTION

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  1. 1 MICROBIAL GENETICS CONJUGATION (MATING) AND TRANSDUCTION (PHAGE-LIKE TRANSDUCING PARTICLES MOVE DNA)

  2. 2 MICROBIAL GENETICS - CONJUGATION MALE, FERTILITY FACTOR, DONOR FEMALE, ABSENCE OF F FACTOR, RECIPIENT F+ MALE, SEX PILI, CONJUGATION BRIDGE, ORIGIN OF TRANSFER, NICKING AT ORIT, TRANSFER OF SINGLE STRAND, COMPLEMENTARY STRAND SYNTHESIS IN MALE AND FEMALE HFR MALE (HIGH FREQUENCY OF RECOMBINATION), INTEGRATION OF TRANSFERRED DNA INTO RECIPIENT CHROMOSOME BY HOMOLOGOUS RECOMBINATION, SELECTION OF RECOMBINANTS F' MALE, ILLEGITIMATE RECOMBINATION, PARTIAL DIPLOIDS

  3. 3 CONJUGATION - MATING OF MALE AND FEMALES E. COLI MALES: FERTILITY FACTOR F PLASMID ~100,000 BASE PAIRS MALE CHARACTERISTICS CONTACT WITH FEMALE DNA DONATION FEMALES: F - DNA RECIPIENT ABSENCE OF F FACTOR F FACTOR - DOUBLE STRAND DNA IS2 INSERTION SEQUENCE 1 F TRANSFER GENES REPLICATION GENES oriT ORIGIN OF TRANSFER

  4. 4 F+ MALE F PILUS BINARY FISSION CHROMOSOME AND F FACTOR DUPLICATED

  5. 5 F FACTOR TRANSFER: F+ x F - F+ AND F+ F+ F - NICK F AT oriT TRANSFER ONE STRAND CONJUGATION BRIDGE NEW SYNTHESIS= F+ F+

  6. APPRECIATE !! TRANSFER GENES OF F FACTOR

  7. APPRECIATE ! F PILUS SECRETION

  8. APPRECIATE ! DNA SECRETION APPARATUS

  9. 6 HFR [HIGH FREQUENCY RECOMBINANT] MALES TRANSFER CHROMOSOME FRAGMENTS FORMING HFR F INTEGRATES INTO CHROMOSOME; F+ BECOMES HFR oriT IS2 F+ MALE PRO+ LAC+ IS2 HOMOLOGOUS RECOMBINATION BETWEEN IS2 oriT IS2 IS2 HFR MALE PRO+ LAC+

  10. 7 HFR x F- RECOMBINANTS HFR PRO+ STRS x F- PRO- STRR RECOMBINANT PRO+ STRR PROLINE F- NEW SYNTHESIS HFR CONJUGATION BRIDGE BREAKS

  11. 8 CONSIDER RECIPIENT CELL ONLY HOMOLOGOUS RECOMBINATION - TWICE LEFT AND RIGHT OF MUTATION LINEAR FRAGMENT DEGRADATION PRO+ STR-R F- RECOMBINANT

  12. 9 DEMONSTRATION GLUCOSE AND STREPTOMYCIN ~108 HFR PRO+ STR-S ~108 F- PRO- STR-R MIX ~108 HFR ~108 F- PRO+ STR-R RECOMBINANTS

  13. 10 HFR CAN EXCISE F FACTOR PRECISELY TO REGENERATE F+ MALE IS2 IS2 HFR IS2 IS2 HOMOLOGOUS RECOMBINATION BETWEEN IS2's IS2 F+ MALE IS2

  14. 11 F' MALE : F PLUS CHROMOSOME GENE(S) PRO+ HFR LOOPING STR-S PRO+ ILLEGITIMATE RECOMBINATION DELETION PRO+ F' MALE STR-S

  15. 12 F' MATING F' x F- F' + NEW F' PRO+ PRO+ PRO- STR-S STR-R F' STR-S PRO+ F- PRO- STR-R PRO+ PRO+ PRO- STR-S STR-R NEW F' MALE PARTIAL DIPLOID

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