NemOut TM A new nematicide in development for control of reniform and other nematodes in cotton

1. NemOutTMA new nematicide in development for control of reniform and other nematodes in cotton Timothy Johnson Plato Industries, Ltd. Houston, TX

2. Difficulties in managing nematodes in cotton • Multiple species - root knot, reniform, Columbia lance • Soil pests are generally more difficult to manage than foliar pests • Nematodes are difficult to detect and costly to sample • Distributions are typically uneven • Yield losses are often contingent upon additional stress factors

3. Nematode management strategies • Crop rotation – economic and biological limitations • Host plant resistance – slow to develop and bring to market • Chemical and/or biological intervention

4. Nematode management strategies - chemical • Temik® 15G – long time soil insecticide/nematicide • AVICTA® Complete Pak – includes abamectin for nematode control • Vydate® – layby application • Telone® - soil fumigant

5. Nematode management strategies - biological • N-HibitTM – induces plant defense mechanisms

6. NemOutTM • Pathogenic fungus Paecilomyces lilacinus strain 251 • Primarily an egg parasite with lesser activity on juvenile and adult nematodes

7. NemOutTM • Manufactured by ProPhyta Biologischer Pflanzenschutz GmbH of Poel, Germany • Active ingredient is EPA registered for use on bananas, fruiting vegetables and turf. • NemOut is a new formulation specifically for cotton and peanuts and is being developed by Plato Industries, Ltd. of Houston, TX

8. NemOutTM -mode of action • Paecilomyces lilacinus mycelium infecting nematode eggs. ©Rita Holland

9. NemOutTM – development objectives for cotton and peanuts • Use Rates • Water volumes • Application timing and methodology • Compatibility with fungicides

10. NemOutTM – 2006 field trials • Cotton – Mississippi delta • Peanuts – Alabama and Florida

11. DREC, MSU – Trial 1 Cooperator: Dr. J. Smith Original 1X formulation Untreated 2 lbs NemOut IF 4 lbs NemOut IF 8 lbs NemOut IF Temik 15G 5 lb IF Avicta C-P 200 ft. plots x 4 rows 6 reps RCBD Planted 4/19 Harvested 9/20

12. Veris Data Trial 1 Measuring Soil Electrical Conductivity (EC)

13. Pre-treatment Post-treatment NemOut -Trial 1 Reniform nematodes per 500 cc soil

15. NemOutTM – trial 1 harvest GPS linked picker with continuous read out yield monitor

16. DREC, MSU – Trial 2 Cooperator: Dr. G. Scuimbato New 10x formulation Untreated 0.2 lbs NemOut IF 0.4 lbs NemOut IF 0.8 lbs NemOut IF Temik 15G 5 lb. IF Avicta C-P NemOut Seed Trt. 1 lb/100 CWT 2 lb/100 CWT 2 lb/CWT + Temik 2 lb/CWT + Avicta 45 ft. plots x 4 rows 5 reps RCBD Planted 5/4 Harvested 9/26

17. Mixing conidia. Injecting seed with conidia. Treated seed. Preparing seed with NemOut

20. NemOutTM – peanut trial Cooperator: Dr. J. Rich

21. NemOutTM – peanut trial Cooperator: Dr. K. Lawrence

22. NemOutTM – future direction • Complete compatibility studies with seed applied fungicides • Expand geographic area for cotton trials to include both root knot and reniform infestations • Focus on application methodologies to include application through irrigation systems and hopper box applications

23. NemOutTM – future direction • Proceed with evaluating use rates that are economically competitive with commercial standards • Complete the registration process to allow large-scale evaluations in 2007

24. Acknowledgements Mississippi State University – DREC Dr. Jim Smith Dr. Gabe Sciumbato Dr. Pat English Boisee Stokes Troy Gibson University of Florida – Quincy Dr. Jim Rich Auburn University Dr. Kathy Lawrence