1 / 10

Using and Analyzing Fluorescent Proteins: BLAST KEY

Using and Analyzing Fluorescent Proteins: BLAST KEY. 2013 Workshop C: Cloning DNA to Make Proteins Dina N Kovarik, MS, PhD Digital World Biology. Research Questions.

ahanu
Download Presentation

Using and Analyzing Fluorescent Proteins: BLAST KEY

An Image/Link below is provided (as is) to download presentation Download Policy: Content on the Website is provided to you AS IS for your information and personal use and may not be sold / licensed / shared on other websites without getting consent from its author. Content is provided to you AS IS for your information and personal use only. Download presentation by click this link. While downloading, if for some reason you are not able to download a presentation, the publisher may have deleted the file from their server. During download, if you can't get a presentation, the file might be deleted by the publisher.

E N D

Presentation Transcript

  1. Using and Analyzing Fluorescent Proteins: BLAST KEY 2013 Workshop C: Cloning DNA to Make Proteins Dina N Kovarik, MS, PhD Digital World Biology

  2. Research Questions The cloning and protein purification experiments you have been conducting in the laboratory involve mTomato, also called red fluorescent protein (RFP). • Is red fluorescent protein (RFP) related to its famous cousin, GFP? • What other fluorescent proteins, if any, are closely related to GFP and/or RFP?

  3. Comparing eGFP and pLemon-YFP DNA Sequences

  4. Comparing RFP and mGrape-PFP DNA Sequences

  5. Comparing eGFP and RFP DNA Sequences

  6. Comparing eGFP and pLemon-YFP Protein Sequences

  7. Comparing RFP and mGrape-PFP Protein Sequences

  8. Comparing All Fluorescent Proteins:BLAST Scores

  9. All Fluorescent Proteins: Distance Tree of Results

  10. Diversity and Key Mutations

More Related