1 / 19

Heat Stress (HS) in Blueberry Final report 1 1 April, 2013

Heat Stress (HS) in Blueberry Final report 1 1 April, 2013. Temperature Sensor. c yclic nucleotide-gated calcium channel Arabidopsis has two paralogs (CNG2 and CNG4) .

aiko
Download Presentation

Heat Stress (HS) in Blueberry Final report 1 1 April, 2013

An Image/Link below is provided (as is) to download presentation Download Policy: Content on the Website is provided to you AS IS for your information and personal use and may not be sold / licensed / shared on other websites without getting consent from its author. Content is provided to you AS IS for your information and personal use only. Download presentation by click this link. While downloading, if for some reason you are not able to download a presentation, the publisher may have deleted the file from their server. During download, if you can't get a presentation, the file might be deleted by the publisher.

E N D

Presentation Transcript

  1. Heat Stress (HS) in Blueberry Final report 11April, 2013

  2. Temperature Sensor • cyclic nucleotide-gated calcium channel • Arabidopsis has two paralogs (CNG2 and CNG4) AndrijaFinka, America FariniaHenriquezCuendet, Frans J.M. Maathuis, YounousseSaidi,and Pierre Goloubinoffa.. Plasma Membrane Cyclic Nucleotide Gated Calcium Channels Control Land Plant Thermal Sensing and Acquired Thermotolerance. The Plant Cell, Vol. 24: 3333–3348, August 2012.

  3. Conserved Family of Genes

  4. Deletions Grow Slower 20° C Arabidopsis Moss WT CNGC2-/- AndrijaFinka, America FariniaHenriquezCuendet, Frans J.M. Maathuis, YounousseSaidi,and Pierre Goloubinoffa.. Plasma Membrane Cyclic Nucleotide Gated Calcium Channels Control Land Plant Thermal Sensing and Acquired Thermotolerance. The Plant Cell, Vol. 24: 3333–3348, August 2012.

  5. CNGC2-/- Hyper-Thermosensitive Moss HSP promoter + Reporter

  6. More Calcium Enters CNGC2-/- Moss CNGC2-/- WT

  7. 3 Temp-Sensitive Ca2+ Channels Moss CNGC2-/- WT conductance in picosiemens (1 – 100 range)

  8. Prime Plants for Thermotolerance Arabidopsis CNGC2-/- WT

  9. Full HSR at Lower Temps Arabidopsis WT CNGC2-/-

  10. Survive Extreme Heat Treatment CNGC2-/- WT Arabidopsis

  11. Post Temperature Sensor calcium channel in PM (primary pathway) histone sensor in the nucleus unfolded protein sensor (UPR) in the endoplasmic reticulum unfolded protein sensor (UPR) in the cytosol

  12. Seed Germination • tolerant NCED4 allele is responsible for germination at high temperatures in lettuce • encodes 9-cis-epoxycarotenoid dioxygenase4 (1.13.11.51) • Catalyzes the first step of abscisic-acid biosynthesis from carotenoids in chloroplasts, in response to water stress

  13. NCED4 Determines Heat Inhibition wt NCED4 inhib NCED4 heat tolerant seeds heat inhibited seeds tolerant NCED4 inhib NCED4 + RNAi HeqiangHuo, PeetambarDahal, KeshavuluKunusoth, Claire M. McCallum, and Kent J. Bradford.2013 ePublication. Expression of 9-cis-EPOXYCAROTENOID DIOXYGENASE4 Is Essential for Thermoinhibition of Lettuce Seed Germination but Not for Seed Development or Stress Tolerance. www.plantcell.org/cgi/doi/10.1105/tpc.112.108902.

  14. NCED4 Determines Heat Inhibition wt NCED4 inhib NCED4 heat tolerant seeds heat inhibited seeds tolerant NCED4 inhib NCED4 + RNAi HeqiangHuo, PeetambarDahal, KeshavuluKunusoth, Claire M. McCallum, and Kent J. Bradford.2013 ePublication. Expression of 9-cis-EPOXYCAROTENOID DIOXYGENASE4 Is Essential for Thermoinhibition of Lettuce Seed Germination but Not for Seed Development or Stress Tolerance. www.plantcell.org/cgi/doi/10.1105/tpc.112.108902.

  15. HS Pathway • HS response requires heterotrimer of HsfA+B+C • HsfA1 (family) is the master regulator • reverts when cooled and HsfB antagonism wins

  16. HsfA1-independent Pathway heat may be able to activate HS response without HsfA1

  17. genes involved in thermotolerance

  18. List of Genes with 51 SSRs Primer Pairs xn FAD5 scaffold00455x3 ACD2 scaffold00474x3 RBOHB scaffold00539x3 FAD4 scaffold00588x3 BZIP28 scaffold00604x3 BZIP17 scaffold00617x3 IRE1 scaffold00646x3 DREB scaffold00667x3 MS1 scaffold00725x3 BIP2 scaffold00773x3 NCED4 scaffold00790x2 VTC1 scaffold00867x3 CAM3 scaffold00875x3 CDPK 2 scaffold00916x3 ETO1 scaffold00956x3 AXR1 scaffold01037x3 PIPK scaffold01039x3 HSFA1 scaffold01062x3 RBOHF scaffold01076 x1 UBP2 scaffold01164 x3 ABI2 scaffold01374x3 HOT1 scaffold01438x3 ETO2 & 3 scaffold01451x3 UVH3 scaffold01548x2 UVH1 scaffold02430x2 ETO1 #2 scaffold01626x2 FAD2 scaffold01825x2 EIN2 scaffold02031 CD UBP1 scaffold02114x2 FAD7 scaffold02449x1 UTR3 scaffold02795x1 BZIP60 no scaffold NPR1 scaffold12427 x0 WRKY 3 scaffold00003 x 3 RBOHD & E scaffold00029 x 3 BIP1 scaffold00039 x3 MBF1scaffold00048 x3 H2A.Z aka HTA11 scaffold00069 x4 CNGC2 & CNGC4 scaffold00079 x3 CAT1 scaffold00093x3 HSFA2 scaffold00109x3 ETR1 scaffold00112x3 FAD6 scaffold00117x3 HSFB1 scaffold00122 x3 FAD3 scaffold00126x3 PLD scaffold00152 x3 ABI3 scaffold00173x3 UVH6 scaffold00209x3 CAD2 scaffold00210x3 VTC2 scaffold00252x3 NPQ1 scaffold00260x3 ABI1 scaffold00338x3 RBOHA & C & G & H scaffold02317x2

  19. References AndrijaFinka et al. 2012. Plasma Membrane Cyclic Nucleotide Gated Calcium Channels Control Land Plant Thermal Sensing and Acquired Thermotolerance. The Plant Cell. Vol. 24: 3333–3348. HeqiangHuo et al.2013 ePublication. Expression of 9-cis-EPOXYCAROTENOID DIOXYGENASE4 Is Essential for Thermoinhibition of Lettuce Seed Germination but Not for Seed Development or Stress Tolerance. www.plantcell.org/cgi/doi/10.1105/tpc.112.108902. Jane Larkindale et al. 2005. Heat Stress Phenotypes of Arabidopsis Mutants Implicate Multiple Signaling Pathways in the Acquisition of Thermotolerance. Plant Physiology.Vol. 138: 882–897. SachinKotak et al. 2007. A Novel Transcriptional Cascade Regulating Expression of Heat Stress Proteins duringSeedDevelopmentofArabidopsis. The Plant Cell, Vol. 19: 182–195. Ching-HuiYeha et al. 2012. Some like it hot, some like it warm: Phenotyping to explore thermotolerancediversity. Plant Science. Vol. 195: 10-23. Yong-Xiang Lin et al. 2011. Genome-wide identification, classification and analysis of heat shock transcription factor family in maize. BMC Genomics. Vol. 12: 76. Hsiang-chin Liu and Yee-yungCharng. 2012. Acquired thermotoleranceindependent of heat shock factor A1 (HsfA1), the master regulator of the heat stress response. Plant Signaling & Behavior 7(5): 547–550. Jason Argyris, et al. 2011. A gene encoding an abscisic acid biosynthetic enzyme (LsNCED4) collocates with the high temperature germination locus Htg6.1 in lettuce (Lactuca sp.) TheorAppl Genet. Vol. 122:95–108. YounousseSaidi, et al. 2009. The Heat Shock Response in Moss Plants Is Regulated by Specific Calcium-Permeable Channels in the Plasma Membrane. The Plant Cell. Vol. 21: 2829–2843. OndrejKrinke, et al. 2009. Phospholipase D Activation Is an Early Component of the Salicylic Acid Signaling Pathway in Arabidopsis Cell Suspensions. Plant Physiology. Vol. 150, pp. 424–436.

More Related