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Lab 9

Lab 9. The Kidney. Lab 9: The Kidney. Dialysis experiment results and tests for NaCl and albumin ( start dialysis first ) Kidney dissection Urinalysis Interpret dipstick with the help of a chart Normal and abnormal constituents of urine Urinary sediment (pictures +/or slides)

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Lab 9

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  1. Lab 9 The Kidney

  2. Lab 9: The Kidney • Dialysis experiment results and tests for NaCl and albumin (start dialysis first) • Kidney dissection • Urinalysis • Interpret dipstick with the help of a chart • Normal and abnormal constituents of urine • Urinary sediment (pictures +/or slides) • Histology • Kidney #62, Ureter #62, and Bladder #10 • PhysioEx 9 Renal Physiology • Models • Urinary system • Kidney (including nephron and glomerulus models)

  3. Dialysis and Kidney Dissection For each group of 4 students: • Have two students set up the dialysis experiment • Have the other two students start the kidney dissection

  4. Dialysis of Albumin and NaCl • Add albumin & NaCl using separate pipettes • Tie bag • Incubate for one hour • Test beaker water using biuret reagent for protein and silver nitrate for Cl- ions

  5. Kidney section

  6. Kidney Dissection • Make a frontal section and observe visible regions • Save kidneys in a glove; mark with group names

  7. A Human Kidney

  8. The Nephron: Functional Unit of the Kidney

  9. Three Physiological Processes • In the Nephron: 1) glomerular filtration, 2) tubular reabsorption , and 3) tubular secretion • These three processes cooperate to achieve the various functions of the kidney • Different sites different primary functions

  10. Cortex – low power

  11. Cortex – with Glomerular Corpuscles

  12. GlomerularFiltration Membrane Diagram

  13. Glomerular Filtration Membrane

  14. Cortex-Medulla Transition Zone

  15. Renal Medulla – Base of Pyramid

  16. Ureter Histology(retroperitoneal)

  17. Urinary Bladder

  18. Transitional Epithelium

  19. Urinalysis

  20. Calcium Oxalate Crystals

  21. RBCs – Some Crenated

  22. WBCs & Bacteria (Rods)

  23. Yeast

  24. Squamous Epithelial Cells from the Urethra

  25. Spermatozoan

  26. PhysioEx9, Activity 1: Effect of arteriole radius on GFR • Major modifications!

  27. PhysioEx 9, Activity 1: Effect of arteriole radius on GFR • Disregard instructions on Lab 9-15. • Start with an afferent radius of 0.60 mm! • Efferent = 0.30 mm • Decrease afferent radius by 0.5 until it equals 0.35 mm • Start with an afferent radius of 0.50 mm! • Efferent = 0.45 mm • Decrease efferent radius by 0.5 until it equals 0.30 mm • Record data in a modified version of table 3

  28. PhysioEx 9, Activity 1: Effect of arteriole radius on GFR • Table 3 (page Lab 9-20) modifications • Column headings should be: • Afferent arteriole radius (mm) • Glomerular Capillary Pressure (mm Hg) • Glomerular Filtration Rate (ml/min) • Urine Volume (ml) • Last 4 runs, enter efferent instead of afferent radius • Final row will be blank! • Please change table 3 accordingly.

  29. PhysioEx9, Activity 2: The effect of pressure on GFR • Record data in table 4 • Table 4 should be entitled: “The effect of blood pressure…”

  30. Complete data table and homework on pages 9-19 to 9-20. Attach a sketch of the microscopic sediments found in your urine. Construct and properly label six graphs using the data from tables 3 and 4. Instructions for the graphs are on p. 9-20. You can use plotting software, e.g., Excel, if you like. Complete Assignment 9 on MasteringAandP. Homework

  31. Please Leave the Lab Areas Clean • Gloves and tissue scraps to biohazard bucket • Dissecting trays & instruments washed and replaced • Urines flushed down the sink with plenty of water • Urine centrifuge tubes washed, rinsed, and disinfected; then returned to their racks to dry. • Urine cups, dipsticks, & paper towels in regular trash • Used microscope slide cover slips in sharps containers • Plain microscope slides washed and returned to microscope slide boxes • Test tubes washed and returned to racks to dry • Disinfect lab table and sink surfaces

  32. End Lab 10

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