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A. 1.6. 1.4. 1.2. 1.0. gene expression (arbitrary units). 0.8. 0.6. 0.4. 0.2. 0. RA. iHBO. RA. iHBO. RA. iHBO. RA. iHBO. Reelin. Cux2. Nfh. Sox5. B. 1.6. 1.2. gene expression (arbitrary units). 0.8. 0.4. 0. RA. HBO. RA. HBO. RA. HBO. RA. HBO. GOT1. NCAML1.
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A 1.6 1.4 1.2 1.0 gene expression (arbitrary units) 0.8 0.6 0.4 0.2 0 RA iHBO RA iHBO RA iHBO RA iHBO Reelin Cux2 Nfh Sox5 B 1.6 1.2 gene expression (arbitrary units) 0.8 0.4 0 RA HBO RA HBO RA HBO RA HBO GOT1 NCAML1 IL-6 MCP1 Figure S2 Rink et al 2009 C Primer sequences: NF-L: F: 5’-CTTTCGGGATGCCATGCGAC-3’ (neurofilament L) R: 5’-GGCTTCCTTCACCAGCTCCAA-3’ GFAP: F: 5’-CACGTGGAGATGGATGTGGC-3’ (glial fibrillary acidic protein) R: 5’-CAGTTGGCGGCGATAGTCATTA-3’ MCP1: F: 5’-ATGCAGTTAATGCCCCACTC-3’ (monocyte chemoattractant protein 1) R: 5’-TTCCTTATTGGGGTCAGCAC-3’ IL-6: F: 5’-TATGTTCTCAGGGAGATCTT-3’ (interleukin 6) R: 5’-TAGAAACGGAACTCCAGAAG-3’ NCAML1: F: 5’-CAGCCCCTGCCTGCTCAT-3’ (neural cell adhesion molecule 1) R: 5’-CGTCCAGCGGAACTCCACTT-3’ GOT1: F: 5’-CCGATCCCCGCAAGGTTAA-3’ (glutamate oxaloacetate transaminase) R: 5’-CGCGCTAAGAAGTCAGCTCCAAT-3’ p47phox: F: 5’- TTCCCAAATGCAAAGAAACC-3’ R: 5’- AGTTACAGGCCGATGTGACC-3’ Reelin: F: 5’-GCCAACTGGTGGACACTTTT-3’ R: 5’-AGGGTCACCACAGGAAGTGG-3’ Cux2: F: 5’-ATCGGTCAACAGCTCCATTC-3’ (cut-like domain 2) R: 5’-TGCCTCTATGGCCTCAGACT-3’ Nfh: F: 5’-TGAGGAGAAACCCAAAATGG-3’ (neurofilament H) R: 5’-CCGCCATCTAAGTGGTGTTT-3’ Sox5: F: 5’-AACAAGCACAGATCCCCATC-3’ (SRY-related HMG-box 5) R: 5’-GGCTTGTCCTGCAATATGGT-3’