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Lu et al. Figure S2

A. B. WT I26A C61G. C. Input. IP: Flag. Flag-FbxO44 - + + + - + + + - + + + Skp1/Cul1/Roc1 + + + + + + + + + + + +

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Lu et al. Figure S2

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  1. A. B. WT I26A C61G C. Input IP: Flag Flag-FbxO44 - + + + - + + + - + + + Skp1/Cul1/Roc1 + + + + + + + + + + + + HA-BRCA1#1 + - + + + - + + + - + + E2 + + - + + + - + + + - + + - + E2 + + + + + + HA-p27 HA- BRCA1#1 p27 - + + - + + Flag-Skp2 + + + + + + Skp1/Cul1/Roc1 WT I26A C61G Flag-Skp2 - + + Flag-FbxO44 - + + - + + - + + Skp1/Cul1/Roc1 + + + + + + + + + + + + E2 + - + + - + + - + + - + BRCA1#1 -Ub(n) IP: Flag HA-p27-Ub(n) IB: BRCA1 IB: Flag Flag-Skp2 IB: HA Input IB: BRCA1 Flag-FBXO44 IB: Myc Myc-Cul1 Myc-Skp1 Myc-Roc1 Lu et al. Figure S2

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