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Biuret Test

Biuret Test. (Write in glossary): A simple biochemical reaction to detect the presence of protein. A p urple colour shows that protein is p resent , a b lue colour shows that protein is a b sent . Protein Assay. Purpose and goals: To pipette accurately To plot results

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Biuret Test

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  1. Biuret Test (Write in glossary): A simple biochemical reaction to detect the presence of protein. Apurple colour shows that protein is present, a blue colour shows that protein is absent.

  2. Protein Assay Purpose and goals: • To pipette accurately • To plot results • To analyse results

  3. Serial Dilution

  4. Quick Questions • What was the independent variable? • What was the dependent variable? • What were the control variables?

  5. Quick Questions • Independent variable = conc. of protein • Dependent variable = absorbance • Control variables = drops of biuret A B, total volume of solution, time

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