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Standardization of Sample Preparation for NAT

SoGAT XVII May 27, 2004 Dr. Frank Krieg-Schneider Strategic Alliances QIAGEN. Standardization of Sample Preparation for NAT. Standardized systems for sample preparation. Agenda QIAamp DSP Virus kit BioRobot M48 for medium throghput. Manual sample preparation: QIAamp DSP Virus kit.

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Standardization of Sample Preparation for NAT

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  1. SoGAT XVII May 27, 2004 Dr. Frank Krieg-Schneider Strategic Alliances QIAGEN Standardization of Sample Preparation for NAT

  2. Standardized systems for sample preparation Agenda • QIAamp DSP Virus kit • BioRobot M48 for medium throghput

  3. Manual sample preparation: QIAamp DSP Virus kit Features • 500µl sample input volume • Serum or plasma • Viral DNA or RNA purified from virus positive samples • Elution in 20 µl or 60 µl • Amplification using commercial or homebrew assays • Probit analysis to determine 95% cut off

  4. DSP Virus kit: Linear Range

  5. Automated sample preparation on BioRobot M48 • 6–48 samples per run • Serum or plasma • Citrate- or EDTA-preserved • 400 µl sample input volume • Magnetic silica particles

  6. MagAttract Virus M48 ― R&D data • Efficient purification: vDNA & vRNA Typical RNA Virus Typical DNA Virus

  7. MagAttract Virus M48 ― R&D data • Cross-contamination • Alternating +/- samples on worktable • 107 c/ml armored RNA • No false-positives detected • Reproducibility

  8. Standardizing sample preparation in 2004 • QIAamp DSP Virus kit: CE certified in April 2004 • BioRobot M48 for medium throghput in May 2004 • BioRobot MDx: CE certification in Q3/04 • BioRobot EZ1: for low throughputin Q3/04

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