Final Year Project – Seminar Presentation

1. Final Year Project – Seminar Presentation Azizan Mohd. Noor UniKLMICET

2. Why do I need to do well in Presentations? • Phase 1 – 35% • Phase 2 – 30% • Approx. 32.5% of 6 credits; equivalent to 1.95 credits of the total marksin the assessment of the project (6credits) comes from Presentations. • Presentation is only for 30 min each • For a normal course 1.95 credits would be equivalent to approximately 18 h of lectures or 336 h of lab. time; in 14 weeks! • WORTH YOUR WHILE TO DO WELL!

3. Prerequisites for a good seminar presentation • Good research • Good understanding of your research • Well prepared materials

4. Preparation of seminar materials • Suitable for the time allocated ie. 20 min presentationand10 min Q&A • Suitable medium for presentation – Powerpoint being the usual choice • Precise and concise

5. Content of a seminar presentation • Introduction • Objective(s) • Materials and methods • Results and discussion • Conclusion

6. Introduction • A brief description of the background of the research • Current status of knowledge in the area of research • Importance of the research Intro1

7. Poly(3-hydroxyalkanoate) PHA • An intracellular storage material • accumulated by a wide variety • of microorganisms O C C (CH2)n 100-30 000 O R Intro2

8. PHA can be divided into: • Short-chain-length PHA (scl-PHA) eg: 3-hydroxypropionate, 3-hydroxybutyrate, 4-hydroxybutyrate, 3-hydroxyvalerate • Medium-chain-length (mcl-PHA) eg: 3-hydroxyhexanoate, 3-hydrooctanoate, 3-hydroxydecanoate Intro3

9. The type of PHAs produced depends on: • Bacteria strains • Carbon sources • Culture conditions Intro4

10. Some uses of PHAs • Degradable packaging for consumer products eg. bottles, bags, films • Drug-release matrix devices • Starting material for the synthesis of enantiomeric pure chemicals • Paint industry – as a binder Intro5

11. To screen for local PHA producers • To optimize the culture conditions • To manipulate the molecular • weight of the polymer(s) Objectives

12. Materials and methods • Main methods used • Concise – use diagrams or flow-charts if possible Materials n methods 1

13. Procedure Soil samples (1 g) obtained from various locations were suspended in 10 ml of sterile distilled water. The suspensions were then plated out on nutrient agar and incubated at room temperature. Colonies producing biopolymer were identified by the flourescent orange colour formed upon flooding with Nile Blue A. To assess the efficacy of PHA production by the various isolates, the colonies were grown in semi-synthetic liquid medium with glucose as the C-source. The cultures were incubated aerobically for 7 days after which the PHA produced were determined using a gas-liquid chromatograph. Materials n methods2

14. Procedure Soil samples (1 g) obtained from various locations were suspended in 10 ml of sterile distilled water. The suspensions were then plated out on nutrient agar and incubated at room temperature. Colonies producing biopolymer were identified by the flourescent orange colour formed upon flooding with Nile Blue A. To assess the efficacy of PHA production by the various isolates, the colonies were grown in semi-synthetic liquid medium with glucose as the C-source. The cultures were incubated aerobically for 7 days after which the PHA produced were determined using a gas-liquid chromatograph. Materials n methods3

15. Procedure Screening Growth of USM4-55 in specific medium Aerobic fermentation (shake flask) Cell harvesting Cell drying (freeze-drying) GC polymer extraction Molecular weight analysis Materials n methods4

16. Results • Very improtant • Concise • Arranged according to the objectives of the seminar • Table, graphorphoto? Results 1

17. C/N(mol/mol) Cell dry weight (g/l) Polyester content (wt%) Polyester composition 3C4 3C6 3C10 3C8 3C12 3C12:1 3C14 10 4.50 25 22 2 24 26 15 2 10 15 3.88 26 23 2 24 25 10 1 14 20 2.92 37 14 1 31 32 9 1 12 30 1.60 43 19 1 24 30 12 1 13 3C4 - 3-hydroxybutyrate, 3C6 - 3-hydroxyhexanoate, 3C8 - 3-hydroxyoctanoate, 3C10 - 3-hydroxydecanoate, 3C12 - 3-hydroxydodecanoate, 3C12:1 - 3-hydrododecenoate, 3C14 - 3-hydroxytetradecanoate 40 1.25 50 8 1 28 32 12 1 18 Effect of C/N molar ratio on production of polymer from oleic acid by USM4-55 Results 2

18. C/N(mol/mol) Cell dry weight (g/l) Polyester content (wt%) Polyester composition 3C4 3C6 3C10 3C8 3C12 3C12:1 3C14 10 4.50 25 22 2 24 26 15 2 10 15 3.88 26 23 2 24 25 10 1 14 20 2.92 37 14 1 31 32 9 1 12 30 1.60 43 19 1 24 30 12 1 13 3C4 - 3-hydroxybutyrate, 3C6 - 3-hydroxyhexanoate, 3C8 - 3-hydroxyoctanoate, 3C10 - 3-hydroxydecanoate, 3C12 - 3-hydroxydodecanoate, 3C12:1 - 3-hydrododecenoate, 3C14 - 3-hydroxytetradecanoate 40 1.25 50 8 1 28 32 12 1 18 Effect of C/N molar ratio on production of polymer from oleic acid by USM4-55 Results3

19. Polymer molecular weight profile Results 4

20. Optimisation of glucoamylase production by Aspergillus niger Results 5

21. Microorganism • Pseudomonas sp. USM4-55 • was locally isolated from the • soil. It was identified by using • API20E. • Ability to produce biopolymer • detected using Nile-Blue-A • staining method and gas • chromatographic analysis. Results 6

22. P(3HB) mcl-P(3HA) Results 7

23. Conclusion • GC is still the better method for screening of new PHA producers • USM4-55 produces both scl- and mcl-PHA • USM4-55 can use both glucose and oleic acid to produce PHA • Polymer accumulation is highly stimulated by nitrogen and oxygen limitation • Polymer molecular weight can be manipulated by adding certain chemical compounds

24. Effective seminar presentation • Have a good understanding of the research carried out • Confidence and poise • Good English • Address the audience • Adhere to the time allocated • Practise!!