Koch’s Investigation of Anthrax

1. Koch’s Investigation of Anthrax

2. Anthrax that was killing cows and sheep. Koch was curious about the cause of the disease. Koch’s Experimental Step 1 In your science notebook, describe what Koch did in his first step.

3. Koch’s Observation In your science notebook, record your observations.

4. Koch’s Experimental Step 2: Koch’s Observation: What can you infer from the observations of sick and healthy cows? Record your inference in your science notebook.

5. Koch’s Inference: the “rods” and “threads” cause anthrax. What would be the next experimental step? Record the experimental step in your science notebook.

6. Koch’s Experimental Step 3: Koch’s Observation: What can you infer from this observation? Record your inference in your science notebook.

7. Koch’s Inference: the mouse died of anthrax. What would be the next experimental step? Record the experimental step in your science notebook.

8. Koch’s Experimental Step 4: tissue and blood from dead mouse spleen Koch’sObservation: What can you infer from this observation? Record your inference in your science notebook.

9. Koch’s Inference: the blood from the cow caused anthrax in the mouse. What would be the next experimental step? Record the experimental step in your science notebook.

10. Koch’s Experimental Step 5: Blood from cow died from anthrax Koch’s Observation: Blood and tissue from dead mouse spleen What can you infer from this observation? Record your inference in your science notebook.

11. Koch’s Inference: the “rods” and “threads” are alive. Koch’s Experimental Step 6: After transferring one drop of culture to a new slide and letting it sit each day for 8 days, Koch had a purified culture. What would be your next experimental step? Record the experimental step in your science notebook.

12. Koch’s Experimental Step 7: Purified culture from Step 6 What can you conclude about the cause of anthrax? Record in your science notebook.