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Mouthwash A Closer Look at Biofilms

Mouthwash A Closer Look at Biofilms. Picture taken from: http://www.ehponline.org/docs/1998/106-12/innova-biofilmB.JPG. Introduction What we want? Mechanism of action of S. mutans Methods Ways to block adhesion Discussion. Introduction. What we want?.

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Mouthwash A Closer Look at Biofilms

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  1. MouthwashA Closer Look at Biofilms Picture taken from: http://www.ehponline.org/docs/1998/106-12/innova-biofilmB.JPG

  2. Introduction • What we want? • Mechanism of action of S. mutans • Methods • Ways to block adhesion • Discussion Introduction What we want? • Target the biofilm, before it develops. • As mentioned last week, biofilms on our teeth are formed between the interaction with S. mutans. • If we prevent this interaction, we can prevent biofilm formation. • Without a biofilm, bacterial species in our mouth are vulnerable and can hopefully be destroyed with simpler methods. 2

  3. Introduction • What we want? • Mechanism of action of S. mutans • Methods • Ways to block adhesion • Discussion Introduction Mechanism of action of S. mutans Step 1: pac Step 2: Expression of the cell surface adhesion antigen PAc 3

  4. Introduction • What we want? • Mechanism of action of S. mutans • Methods • Ways to block adhesion • Discussion Methods Ways to block adhesion • Design a strain of S. lactis with the pac gene. • Binding of both S. lactis and S. mutans would force competitive binding. • If possible, we can have our strain grow at a wanted rate that would enable it to block off S. mutans. 4

  5. Introduction • What we want? • Mechanism of action of S. mutans • Methods • Ways to block adhesion • Discussion Methods Ways to Block Adhesion • Use antibodies • 1 Problem: How can we connect this to synthetic biology? • To my knowledge, I haven’t seen Ab’s in the registry, so if we could make it work, adding new parts is always a plus. • Design our Ab’s to bind to the PAc antigen and destroy S. mutans with WBC. 5

  6. Introduction • What we want? • Mechanism of action of S. mutans • Methods • Ways to block adhesion • Discussion Methods Ways to Block Adhesion Binding of S. mutans Binding of Ab Binding of Ab, with neutrophil ready to destroy 6

  7. Introduction • What we want? • Mechanism of action of S. mutans • Methods • Ways to block adhesion • Discussion Discussion What Next? • Now that we hopefully prevented biofilm formation, we must look on how long this will last? • Still need to have our strain smell good, taste good, out compete S. mutans, and only have to use once a week or less. • Possibily use one strain of S. lactis for biofilm prevention, and use E. coli for smell, taste, etc. • Would it be ideal to use 2 strains in our mouthwash or toothpaste? 7

  8. References • Iwaki M, Okahashi N, Takahashi I, Kanamoto T, Sugita-Konishi Y, Aibara K, and Koga T. Oral immunization with recombinant Streptococcus lactis carrying the Streptococcus mutans surface protein antigen gene. Infect Immun 1990 Sep; 58(9) 2929-34. pmid:2117575. • Kruger C, Hu Y, Pan Q, Marcotte H, Hultberg A, Delwar D, van Dalen PJ, Pouwels PH, Leer RJ, Kelly CG, van Dollenweerd C, Ma JK, and Hammarstrum L. In situ delivery of passive immunity by lactobacilli producing single-chain antibodies. Nat Biotechnol 2002 Jul; 20(7) 702-6. doi:10.1038/nbt0702-702 pmid:12089555

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