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His-SUMO1

Supplemental Fig.1. a. b. V. PML. HA-PML. -. +. +. +. +. His-SUMO1. +. +. -. +. -. +. ++. +. ++. His-SUMO2. +. +. +. +. +. GST-SUMO1. -. -. -. -. +. +. +. -. +. Flag-c-Jun. Mdm2- SUMO2. Ni beads. WCL. Mdm2. c-Jun-His -SUMO1. 75. Ni beads. 1. 2. 3.

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His-SUMO1

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  1. Supplemental Fig.1 a b V PML HA-PML - + + + + His-SUMO1 + + - + - + ++ + ++ His-SUMO2 + + + + + GST-SUMO1 - - - - + + + - + Flag-c-Jun Mdm2- SUMO2 Ni beads WCL Mdm2 c-Jun-His -SUMO1 75 Ni beads 1 2 3 4 50 c 37 His-SUMO1 c-Jun IB c-Jun- SUMO1 HA-PML - + - + 75 100 50 c-Jun- SUMO1 75 c-Jun WCL 37 PML- SUMO1 50 100 c-Jun 75 PML IB 37 GFP 1 2 3 4 1 2 3 4 5

  2. Supplemental Fig.2 a PML 150 150 100 PML 75 100 PML 50 75 actin 37 * 25 50 15 coomassie blue staining Western blot b TRIM27 Mdm2 - 100 - 75 100 Mdm2 75 Trim27 * - 50 c GST-TRIM27 GST-PML GST GST-TRIM27 GST-PML GST 1 2 3

  3. Supplemental Fig. 3 a Trim22S Trim22L Trim11 Trim22L V Trim27 V Trim39 His-SUMO1 + + + - + + + - + - + + + + HA-Mdm2 + - + + - + + + + + - + + - 150 Mdm2- SUMO1 Ni beads 100 100 Mdm2 WCL Actin GFP b Trim27 V Trim24 Trim25 V Trim18 Trim1 V + + + ++ + ++ + ++ + ++ + ++ + ++ 150 Ni beads Mdm2- SUMO1 100 100 Mdm2 WCL Actin GFP c UbcH5a - + + + TRIM27 + + + - 82 p53-Ub 64 p53 p53 1 2 3 4

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