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Previous FCRG Study looking at common sorter contaminants

Sheath Contamination Survey: An Examination of Common (Though Not Necessarily Best) Laboratory Practices A Study of the Flow Cytometry Research Group (FCRG).

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Previous FCRG Study looking at common sorter contaminants

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  1. Sheath Contamination Survey: An Examination of Common (Though Not Necessarily Best) Laboratory PracticesA Study of the Flow Cytometry Research Group (FCRG) Dave Adams, University of Michigan; Alan Bergeron, Dartmouth College; Laura Blunk, Stowers Institute for Medical Research; Kathleen Brundage, West Virginia University; Karen Clise-Dwyer, MD Anderson Cancer Center; Matt Cochran, University of Rochester Medical Center; Monica DeLay, Cincinnati Children’s Hospital; Roxana Del Rio Guerra, University of Vermont; Maris Handley, Massachusetts General Hospital; Peter Lopez, New York University Langone Medical Center; E. Michael Meyer, University of Pittsburgh Cancer Institute; Alan Saluk, The Scripps Research Institute EB: Frances Weis-Garcia

  2. Previous FCRG Study looking at common sorter contaminants • Develop effective endotoxin cleaning procedure • Test for additional contaminants • Poll SRLs beyond the FCRG and evaluate common practices

  3. The Callout • Survey Monkey Platform • Advertised on PUCL mailing list and Google+ Cytometry Community • Bribed with chance to win Starbucks! • Participants asked about their testing procedures, operating procedures and maintenance procedures • Participants asked to submit samples for testing

  4. How’d We Do? • 61 Respondants • 3 from Non-SRLs • 106 Sorters Described • Several Lessons About How Not to Ask a Question in a Survey • 3 Happy Coffee Drinkers

  5. Instrumentation Quick Facts • Of the 106 cell sorters described in the survey: • 54 are housed within Biosafety Cabinets • 55 are operated solely by laboratory staff while the remaining 51 allow trained users to operate the instrument • Average reported instrument usage is 76-100% capacity though that number falls to 51-75% as the number of sorters within the facility increases. • Sheath Sources break down as follows: • 51% of respondents use a commercial 1x saline product • 35% use a commercial concentrate saline product which is then diluted in-house • 14% manufacture their saline in-house

  6. Testing Frequency

  7. Testing Location

  8. User Operation

  9. Bacteria Testing

  10. RNase Testing

  11. Where to From Here? • Nearly half the survey respondents do not perform any proactive sheath testing and yet over half the received samples tested positive for bacterial/fungal contamination. • The prevalence of RNase in our testing pool was low, but it is worth noting that both positive tests were recorded in facilities that do no RNase testing. • Survey results at this point are limited and additional responses will present a better idea of current standard practices.

  12. Acknowledgements We would like to thank all the individuals who answered our survey and provided samples for our study. Follow the QR code to The current survey Follow the QR code to The current survey

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