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Point of Care Testing pH Testing For questions please call 272-0980. Overview. pH paper is impregnated with an indicator dye. After a sample of unknown pH is applied to the paper the color changes in response to the change in hydrogen ion concentration.
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Point of Care Testing pH Testing For questions please call 272-0980
Overview • pH paper is impregnated with an indicator dye. • After a sample of unknown pH is applied to the paper the color changes in response to the change in hydrogen ion concentration. • It is used to measure the pH of bodily fluids, including vaginal and GI. • pH paper should be stored at room temperature, out of direct sunlight. • pH paper is stable until the expiration date printed on the outer box carton.
Quality Control • Quality Control is performed by the Point of Care Office when a new shipment or new lot number is received. • Prior to each patient test the paper should be inspected for discoloration. • If the color or appearance of the pH paper is not correct discard the paper and open a new box.
Specimen Collection • Collect specimen according to clinical protocol • Specimens are stable for 2-5 minutes at room temperature. • Exposure to air longer than this time may cause a change in pH. • Contamination with blood will interfere with the reading. Blood specimens should be read with caution, as it is difficult to interpret the color reaction.
Patient Testing • Remove approximately 3 inches of pH paper from the dispenser. • Touch the pH paper to the sample. • Compare color change to the color chart provided. • Normal vaginal pH is acidic (below 7.0). pH above 7.0 (basic) indicates the presence of amniotic fluid. • The normal reference range of various body fluids is dependant upon the site. • Normal pH of gastric fluid for a patient who has fasted for at least 4 hours usually has a pH range of 1-4. • Fluid from nasal/oral intestinal tube of a fasting patient usually has a pH >6. • A patient with continuous tube feeding may have pH of 5 or greater. • The pH of pleural fluid from the tracheobronchial tree is generally >6. • Tears have a normal pH of 7-8.
Skills Test • Perform a test with the 2 samples provided and write you results down on your answer Sheet. • Note to UBE: provide the staff with a lemon juice (PH 1-2) and a tap water sample (6-7) for the skills test.
Skills Test and On-line Competency. • Your UBE will assess the results in Learning Central • You must also complete the on-line competency – POC 10. • Note to UBE: The use of this story board is only approved for 6 month or annual competencies. Do not use for initial training.