1 / 1

PC12 α 2 cells

Project workflow. Behavioral tests. Immunohistochemical analysis of neurogenesis. Chronic mild stress (uCMS). Animal sacrifice. 1 st Team Workpackage #1. Dissection of brain regions. 3 wks. Pharmacological treatments 4 wks. BrdU. Monoamines determination. Plasticity related

amethyst-day
Download Presentation

PC12 α 2 cells

An Image/Link below is provided (as is) to download presentation Download Policy: Content on the Website is provided to you AS IS for your information and personal use and may not be sold / licensed / shared on other websites without getting consent from its author. Content is provided to you AS IS for your information and personal use only. Download presentation by click this link. While downloading, if for some reason you are not able to download a presentation, the publisher may have deleted the file from their server. During download, if you can't get a presentation, the file might be deleted by the publisher.

E N D

Presentation Transcript

  1. Project workflow Behavioral tests Immunohistochemical analysis of neurogenesis Chronic mild stress (uCMS) Animal sacrifice 1st Team Workpackage #1 Dissection of brain regions 3 wks Pharmacological treatments 4 wks BrdU Monoamines determination Plasticity related gene expression( qRT-PCR ) Immunohistochemical analysis of neuroprotection/apoptosis with in situ hybridization Hippocampus, amygdala, prefrontal cortex , anterior cingulate cortex , hypothalamus, parietal, temporal lobes 2nd Team Workpackage #2 Analysis of apoptosis with in situ hybridization Knock-down or overexpression of β-arrestins 3rdTeam Workpackage #3 PC12α2cells Analysis of neuronal differentiation, apoptosis and signal transduction Hippocampus, amygdala, prefrontal cortex , acc , hypothalamus Plasticity related gene expression (Western blotting) Dominant negative mutant or overexpression ofNrf2 & CREB

More Related