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A new strategy to protect flowers from freezing stress

A new strategy to protect flowers from freezing stress. Liao Pan ( 廖攀 ), Chen Qin-Fang ( 陳琴芳 ), Chye Mee-Len ( 蔡美蓮 ) Wilson & Amelia Wong Professor in Plant Biotechnology 黃乾利辛烱僖基金教授席 ( 植物生物技術學 ) School of Biological Sciences , The University of Hong Kong

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A new strategy to protect flowers from freezing stress

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  1. A new strategy to protect flowers from freezing stress Liao Pan (廖攀), Chen Qin-Fang (陳琴芳), Chye Mee-Len (蔡美蓮) Wilson & Amelia Wong Professor in Plant Biotechnology 黃乾利辛烱僖基金教授席 (植物生物技術學) School of Biological Sciences, The University of Hong Kong http://pcp.oxfordjournals.org/content/early/2014/02/19/pcp.pcu037

  2. Significance: flowers develop into fruits and seeds (food) Protected Freeze-damaged Fruit cropsFlowersDamaged flowers LOSS FOOD

  3. ACYL-COA-BINDING PROTEIN6 (ACBP6) (醯基輔酶A結合蛋白6) • A cold-inducible protein • Expressed in both rosettes and flowers • Rosettes = vegetative tissues • Flowers = reproductive tissues Arabidopsis Chen et al., 2008, Plant Physiol 148: 304-315

  4. Rosettes Chye et al (2013) US Patent No. 8,378,172 ACBP6 overexpression in transgenic Arabidopsis (轉基因擬南芥) enhances freezing tolerance in rosettes OE = overexpression lines; 3 OE lines were used to test reproducibility of results

  5. Flowers Freezing treatment on Arabidopsis flowers 1 2 4oC 3 days -7oC 1 hour 3 Harvest flower samples 4 4oC 12 hours ACBP6-overexpressors (OE) Wild type (WT)

  6. ACBP6-overexpressing flowers are freeze-tolerant Transformed Arabidopsis flower overexpressing ACBP6 (ACBP6-OE) shows intact petals (right) while the control succumbs to freezing stress (left). (-7oC 1 hour) flowers with intact petals Liao et al. 2014, Plant & Cell Physiol

  7. Why are ACBP6-OE flowers freezing tolerant? After freezing treatment, phosphatidylcholine (PC) (卵磷脂) accumulates (25-34% > WT) increasing membrane fluidity, enhancing freezing tolerance. PC increases membrane fluidity Liao et al. 2014, Plant & Cell Physiol

  8. Why are ACBP6-OE flowers freezing tolerant? Plastid-specific membrane galactolipids (nmol mg-1) 質體特有的光合膜半乳糖脂（納摩爾每毫克） After freezing treatment, Monogalactosyldiacylglycerol (MGDG) (單半乳糖二醯甘油酯)accumulates [(MGDGOE-MGDGWT)/MGDGWT = 22-34% > WT] enhancing freezing tolerance. Liao et al., 2014, Plant & Cell Physiol

  9. Conclusion ACBP6 can be used to protect flowers (besides rosettes) from freezing stress Potential applications Flower protection in • agriculture (fruits, seeds) • floriculture (cultivated blooms) • cold region (citrus)

  10. Research Output http://pcp.oxfordjournals.org/content/early/2014/02/19/pcp.pcu037 P Liao, Q Chen, ML Chye (2014) Transgenic Arabidopsis Flowers Overexpressing Acyl-CoA-Binding Protein ACBP6 are Freezing Tolerant. Plant & Cell Physiology doi:10.1093/pcp/pcu037 US Patent No. 8,378,172

  11. Acknowledgements Funded by Wilson & Amelia Wong Endowment Fund (Plant Biotechnology) The University of Hong Kong (CRCG 104001061) Thank you for your attention

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