Web-Based Activity: Guided Notes on Enzymes

1. Web-Based Activity:Guided Notes on Enzymes • Go to “LewPort enzymes” (first link on google) http://www.lew-port.com/10712041113402793/lib/10712041113402793/Animations/Enzyme_activity.html • Read aloud the blue text on each slide • Use this info to fill in the Notes column on the back of your worksheet • Then use the animations on the Enzyme Menu to fill in drawings or descriptions in the Evidence column • We will do the first one together… • You will work quietly w/ your assigned partner to complete the rest. • Objectives for Class: • Describe what an enzyme does and how it works • Explain why an enzyme’s shape is so important

2. Chemical Reaction Vocabulary: • Chemical Reaction: • Process that changes or transforms one substance into another • Either to build something from smaller pieces – Synthesis • Or to break something down – Decomposition • Ex: glucose + O2 CO2 + H2O + ATP energy + heat • Enzyme: • A protein that speeds up chemical reactions in living things (the “catalysts” in our cells and bodies) • Objectives for Class: • Describe and illustrate chemical reactions, reactants, products, and enzymes • Explain how enzymes work to catalyze reactions • Use a knowledge of enzymes to predict what will happen in tomorrow’s lab

3. Chemical Reaction Vocabulary continued: • Reactants: • the starting materials in a chemical reaction • (also known as substrates) • Products: • substances that are produced in the chemical reaction (the end result) • Objectives for Class: • Describe and illustrate chemical reactions, reactants, products, and enzymes • Explain how enzymes work to catalyze reactions • Use a knowledge of enzymes to predict what will happen in tomorrow’s lab

4. Example: Lipid Digestion The enzyme lipase helps to break down lipids into their building block molecules (fatty acids and glycerol) Enzyme – Reactant (aka Substrate) – Products – Lipase Lipids Substrate/ Reactant Products Fatty acids and glycerol Lipase Lipids Fatty acids + glycerol Enzyme helps substrate turn into products • Objectives for Class: • Describe and illustrate chemical reactions, reactants, products, and enzymes • Explain how enzymes work to catalyze reactions • Use a knowledge of enzymes to predict what will happen in tomorrow’s lab

5. Example: Lipid Digestion Which enzyme is best structured to help break down lipids? Why? 1 2 3 • Objectives for Class: • Describe and illustrate chemical reactions, reactants, products, and enzymes • Explain how enzymes work to catalyze reactions • Use a knowledge of enzymes to predict what will happen in tomorrow’s lab

6. Example: Lipid Digestion Which enzyme is best structured to help break down lipids? Why? The SHAPE of an enzyme must match the SHAPE of its specific substrates! 2 • Objectives for Class: • Describe and illustrate chemical reactions, reactants, products, and enzymes • Explain how enzymes work to catalyze reactions • Use a knowledge of enzymes to predict what will happen in tomorrow’s lab

7. Enzyme Lab: Starch DigestionSTARCH GLUCOSE salivary amylase • Procedure • Prediction: • Results: How will we know? *Stir the starch well before using it! Starch & Water Starch & Water & Enzyme (salivary amylase) 1 2 Which tube will contain glucose after 10 minutes? Fill in the Prediction row on your lab table right now. Benedict’s test

8. Enzyme Substrate Products

9. Applying Heat or Acid to Enzymes…

10. Denaturing Enzymes When an enzyme is denatured, it… • Becomes misshapen and deformed • Loses its original shape • Becomes unable to speed up the reaction it is designed for, because its shape no longer matches its substrate