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ISTA – Variety Committee

Day, 19 June 2010 Agenda Point (16:00-17:00) . ISTA – Variety Committee. ... the Technical Committee for Species and Variety Testing Report of … Activities Working program Special projects Berta Killermann. Chair 1 Berta Killermann DE-Germany

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ISTA – Variety Committee

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  1. Day, 19 June 2010 Agenda Point (16:00-17:00) ISTA – Variety Committee • ... the Technical CommitteeforSpeciesandVarietyTesting Report of … • Activities • Working program • Special projects Berta Killermann

  2. Chair 1 Berta Killermann DE-Germany Vice-chair 2 Ana Laura Vicario AR-Argentina Members 3 Emanuela Casarini IT-Italy 4 Rainer Knoblauch DE-Germany 5 Kae-Kang Hwu TW-Taiwan 6 Cheryl Dollard CA-Canada 7 David Zhang FR-France 8 Chiara Delogu IT-Italy 9 Ulf Kjellström SE-Sweden 10 Eszter Timar HU-Hungary 11 Jerzy Drzewiecki PL-Poland 12 Hedwich Teunissen NL-Netherland 13 Savitha H. Reddy IN-India 14 Kalyn Brix-Davis US-United States 15 Wendy Carnegie CA-Canada 16 Michael Lovelace US-United States Committee members

  3. Leader 1 Emanuela Casarini IT-Italy Group leader Co-workers 2 Ana Laura Vicario AR-Argentina soybean 3 Kae-Kang Hwu TW-Taiwan rice 4 Cheryl Dollard CA-Canada 5 CesarOlsina AR-Argentina 6 Daniel Perry CA-Canada wheat 7 Marie-Jose Cote CA-Canada 8 Elisa Vieira BR-Brazil 9 Kalyn Brix-Davis US-United States 10 Kunusoth Keshavulu IN-India 11 Eun-HeeSohKR-Korea 12 Kim KenwardCA-Canada 13 Benjamin Kaufman US-United States maize 14 ChristelleForzy FR-France 15 Chiara Delogu IT-Italy 16 Fengge Wang CN-China 17 GasparMalone AR-Argentina Working Group – DNA-based methods

  4. 2009 • First ISTA Workshop on Molecular Markers forVariety Identity andPurityatthe University in Bologna, Italy (June 2009) • ComparativeTests (microsatellites - SSRs) • 3rd CT formaize, wheat, soybeanandricewererun • 2010andlater • Comparative Tests (microsatellites , SSRs) • 4th CT forsoybeanandwheatarerunning • First PT for DNA-basedmethodsisplannedformaize (underdiscussion) • handlingofreference-material? • performanceofthe PT? (a classical PT or a performancebasedapproach) • Development of an accreditationstandard (including PTs) • a classicalaccreditationstandard • performancebasedapproach, likefor GMO testing Activities - WG DNA-based methods

  5. Leader 1 Chiara Delogu IT-Italy Co-workers 2 Rainer Knoblauch DE-Germany 3 Jerzy Drzewiecki PL-Poland 4 Savitha H. Reddy IN-India 5 Maria Alicia Loray AR-Argentina 6 Kalyn Brix-Davis US-United States 7 Kunusoth Keshavulu IN-India 8 Ravi Age IN-India 9 Yujia Wu US-United States 10 Berta Killermann DE-Germany Working Group – electrophoresis ! Wearelookingforfurtherco-workers !

  6. 2009 • Comparative Tests • Improvement of ISTA A-PAGE method for the verification of Triticum • Test leader Ana Laura Vicario (Argentina) • Design 3 wheat samples (ISTA method) • 3 wheat samples (in-house method) • Participating labs Rainer Knoblauch - LTZ - Germany • M. Alicia Loray - INASE - Argentinia • Berta Killermann - LfL - Germany • Chiara Delogu - ENSE - Italy • Trevor Mazutinec - Bio Vision Seed Lab - Canada Silvia Zanetti - ART - Switzerland • Jerzy Drzewiecki - IHAR - Poland • Status quo data analysis (STACOM) • forward a Rules proposal in 2010 Activities – WG electrophoresis

  7. 2010 • Comparative Tests • SDS-PAGE for the discrimination of Triticum and xTriticosecale • Test leader Chiara Delogu (ENSE, Italy) • Participating labs we are looking for • Status quo test plan and the technical protocol will be presented and discussed in the open VARCOM meeting • IEF for the discrimination of Triticum and xTriticosecale • Test leader not known at present • Status quo under discussion • SDS-PAGE, IEF for the discrimination of Festuca and Poa • Status quo under discussion Activities – WG electrophoresis

  8. 2010 • ISTA Pre-Congress Workshop on SpeciesandVarietyTesting – electrophoresis • (Federal Plant Variety Office, Hanover, LowerSaxony, Germany) Activities – WG Electrophoresis

  9. Activities – WG Electrophoresis

  10. Activities – WG Electrophoresis

  11. Leader 1 Michael Lovelace US-United States Co-workers 1 Benno Voit DE-Germany 3 Wendy CarnegieCA-Canada 4 Berta KillermannDE-Germany 5 Gerry HallUK-United Kingdom 6 ... Working Group – conventional methods ! Wearelookingforfurtherco-workers !

  12. 2010 and later • Engagement in this field of work is of less interest • old fashioned methods but we urgently need this methods • Change in the leadership • where should we focus our activities in future ? • how can we handle this ? • how should we organize this working group ? • What can be done – how can we reach these aims? • developmentof a questionnaireconcerning the need and development for methods of variety control in laboratories and/or in field plots • catch newchallenges (look for methods for testing herbicide tolerances on seedlings by means of germination tests - greenhouse trials) • comparativetests • ... Activities – WG conventional methods

  13. Establishment of a databasefor an inventoryofmethodsforspeciesandvarietytesting • electronical database (highly sophisticated) • working sheets (ring binder) • Revision of the existing four handbooks with the aim to have only one handbook in loose-sheet format with 4 – 5 chapters and a harmonisation in the method description • Electrophoresis Testing • Rapid Chemical Identification Techniques • Growth Chamber – Greenhouse Testing Procedures: Variety Identification • Laboratory Tests for Variety Determination with fungal Pathogens • DNA-based Methods (new capter?) • Organisation offurtherISTA Workshops on Species and Variety Testing • list of possible local organizers (homepage) • list of lecturers (homepage) • safe time and money Projects for the future

  14. Can yougivesupport (documents, statistics) forconducting an in-houseproficiencytestround on hybrid puritytesting (06/2009 India) Assistance in testingmaizeseedintendedforexport, in thecasewhenitis not processed (07/2009 Serbia) Assistance forbying an appropriateequipmentfor IEF (07/2009 Czech Republic) Informationsaboutthestatus quo andthefutureaimsabouttheuseof DNA markertechnology (SSR, etc.) for hybrid puritytestingandwhyonly IEF methodsare incorporated in the Rules – don`tyoufeelthatwiththerecentadvancementsandautomation DNA technologiescouldbemore rapid, reliableandeconomic? (08/2009 India) Assistance forbyingthecorrectchemicalsfor A-PAGE (11/2009 Czech Republic) Howcanweanalysethepurityofricevarieties? (11/2009 Uganda) Are methodsexistingtodifferentiatevarietiesofsoybeanseed lots relatedtoseedlingorhypocotyllength? (12/2009 Argentina) Howcan I testthemeasurementuncertainty in electrophoresismethods? (02/2010 Serbia) Howcan I become a memberofthevarietycommittee, especially in theworkinggroup DNA-basedmethods? Requests in 2009 / 2010

  15. Michael Lovelace Chiara Delogu Ana Laura Vicario Kalyn Brix Davis Cheryl Dollard Acknowledgement David Zhang Rainer Knoblauch Hedwich Teunissen Emanuela Casarini Kae-Kang Hwu Jerzy Drzewiecki Savitha H. Reddy Eszter Timar Wendy Carnegie Ulf Kjellström Berta Killermann

  16. if you want to become a • member • co-worker of the Variety • Committee Open Meeting of the Variety Committee If you have any questions ideas comments or … please contact any of us or join the open meeting of the Variety Committee • Date: June 19th • Time: 19:00 – 20:30 • Room: Meeting Room 2, 1st Floor • (Isabellensaal, rechts)

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