1 / 23

Chapter Ⅱ

Chapter Ⅱ. Lab equipment & procedures. Microscopes. Light Microscopes 1. Single Lens 2. Compound microscopes eye piece objective Total M Low P 10X 10X 100X High P 10X 43X 430X Oil 10X 97X 970X Resolving Power : 0.2 um ( bare eye resolving power : 0. 1mm).

ankti
Download Presentation

Chapter Ⅱ

An Image/Link below is provided (as is) to download presentation Download Policy: Content on the Website is provided to you AS IS for your information and personal use and may not be sold / licensed / shared on other websites without getting consent from its author. Content is provided to you AS IS for your information and personal use only. Download presentation by click this link. While downloading, if for some reason you are not able to download a presentation, the publisher may have deleted the file from their server. During download, if you can't get a presentation, the file might be deleted by the publisher.

E N D

Presentation Transcript

  1. ChapterⅡ Lab equipment & procedures

  2. Microscopes • Light Microscopes 1. Single Lens 2. Compound microscopes eye pieceobjectiveTotal M Low P 10X 10X 100X High P 10X 43X 430X Oil 10X 97X 970X Resolving Power : 0.2 um ( bare eye resolving power : 0. 1mm)

  3. B. U.V. Microscopes U.V. light Resolving power : 0.1 um C. Fluorescence Microscope Fluorescence dye + U.V. Light eg. TB. Use auramine (yellow dye) D. Phase Contrast Microscope For living material, viscosity differences are increased.

  4. The light microscope

  5. E. Electron Micoscope 1. Transmission EM use a beam of electrons ( very short wavelength) Resolving power : 0.001 um Magnification : 100,000 X 2. Scanning EM electrons strike on the surface (3D)

  6. The electron microscope

  7. How to Prepare Slides 1. Living material Hanging drop technique (for movement) 2. Stain bacteria Basic dye : color on “+” charge eg. methylene blue Acidic dye : color on “-” charge eg. nigrosine

  8. Staining Techniques a. Simple stain b. Gram stain 1884 Christian Gram Crystal violet – primary dye Iodine – mordant 95% ETOH – decolorizing agent Safranin –counter stain c. Acid – fast stain for mycobacteria

  9. The Gram stain (1)

  10. The Gram stain (2)

  11. Preparation of a Pure Culture Colonies : Pure culture techniques 1. Streaking methods 2. Liquid dilution methods Culture medium: carbon, nitrogen, minerals and water - defined medium - undefined medium

  12. Method(cont.) Isolation-pure Culture (Discontinuous Streaking) 1. Sterilize wire loop by holding over flame until it turns bright orange 2. Wait a few seconds, cool loop by lightly touching NA 3. Take bacteria with loop from sample culture and streak on other plate. 4. Cover, label, and tape plates. 5. Put in incubator at 37°C

  13. Method(cont.) Isolation: continuous streaking Do the same procedure are discontinuous streaking except follow a different pattern.

  14. Dilutions

  15. Special media: - carbohydrate media - selective medium : select a certain organisms and inhibit other organisms. eg. Bile salt select Salmonella and Shigella but no E. coli - differential medium eg. Blood agar - enrichment cultures eg. Isolate organisms from environment such as soil or pond water mineral medium + sodium benzoate select Pseudomonas.

  16. Viable counting

  17. Turibdimetric growth measurements(1)

  18. Turibdimetric growth measurements(2)

  19. Turibdimetric growth measurements(3)

  20. Bacterial morphologies (1)

  21. Bacterial morphologies (2)

  22. Bacterial morphologies (3)

  23. Bacterial morphologies (4)

More Related