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Construction and analysis of a genomic DNA library

Construction and analysis of a genomic DNA library. 2-1. Construction of a cDNA library. 2-2. Differences between a genomic and cDNA library. Genomic Library Promoters Introns Intergenic Non-expressed genes. cDNA Library Expressed genes Transcription start sites

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Construction and analysis of a genomic DNA library

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  1. Construction and analysis of a genomic DNA library 2-1

  2. Construction of a cDNA library 2-2

  3. Differences between a genomic and cDNA library Genomic Library Promoters Introns Intergenic Non-expressed genes cDNA Library Expressed genes Transcription start sites Open reading frames (ORFs) Splice points (Not in Chapter)

  4. Purification of mRNA 2-3

  5. Synthesis of cDNA from mRNA 2-4

  6. SfiI digestion sites 2-5

  7. SP Primer XP Primer Is there an insert? A.f. insert 2-6

  8. Life cycle of a bacteriophage 2-7

  9. Constructing a genomic library in phage 2-8

  10. Constructing a genomic library in cosmids 2-9

  11. Constructing a genomic library in YACs 2-10

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