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Transmembrane Signal Transduction Inflammatory mediators

Transmembrane Signal Transduction Inflammatory mediators. Induction of EGFP expression by 1 nM IL-1 in HeLa cells transiently transfected with a pIL8 reporter. Minutes after IL1. IL1. vehicle. EGFP Expression from pIL8-d2-EGFP in ten individual cells following

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Transmembrane Signal Transduction Inflammatory mediators

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  1. Transmembrane Signal Transduction Inflammatory mediators

  2. Induction of EGFP expression by 1 nM IL-1 in HeLa cells transiently transfected with a pIL8 reporter Minutes after IL1 IL1 vehicle

  3. EGFP Expression from pIL8-d2-EGFP in ten individual cells following IL-1 (1 nM) stimulation

  4. RelA Nuclear/CytoplasmicDistribution Response to IL-1

  5. RelA Nuclear/CytoplasmicDistribution Heterogeneity

  6. Kinetic Analysis of IL-1 Induced p65/relA Nuclear Translocation

  7. Normalised average fluorescence of IkBa as a function of time and flow rate

  8. Comments Cell to cell variations in both amplitude and kinetics of signal transduction events cause a significant noise in the system. Obtaining detailed information of regulatory mechanisms at the single cell level requires identification of fundamental controlling parameters. -------------- To use the single cell data as the basis for mathematical modelling of signalling networks. To use the theoretical predictions for improved design of the biological experiment.

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