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Detecting GMO Contaminations in Groceries using Gel Electrophoresis & PCR

This research investigates the presence of genetically modified organisms (GMOs) in grocery food products using gel electrophoresis & PCR techniques. The results of the investigation conducted in Germany and the USA are analyzed, providing conclusive evidence of GMO contamination. Suggestions and next steps for further research are also discussed.

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Detecting GMO Contaminations in Groceries using Gel Electrophoresis & PCR

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  1. GMO Finding geneticalmodified contaminations (GMO) in groceries by using gel electrophoresis & PCR Biotechnologisches Gymnasium Mettnau-Schule, Radolfzell

  2. Structure • Experiment design • Grocery samples tested • 3. Results of the investigation of GMO food • - Germany ( 1st and 2nd analysis) • - USA • 4. Conclusion • 5. Suggestions and next steps

  3. Experiment design • Sample preparation2)PCR • 3)Gel electrophoresis 4)Analysis

  4. Grocery samples (N = 23) 3 11 2

  5. Results of the investigation of GMO in food origin GMO primer plant primer result valid 1st analysis Germany tofu 1 - - - +1) tofu 2 - - - +1) soy meal - - - - corn meal - + - + hamburger - - - +1) tobacco - - - - dog‘s feed - - - +1) popcorn Seeberger - + - + negative c. - - - -2) positive c. + + + + 1 = no chlorophyll 2 = DNA was digested

  6. 2nd analysis GMO primer plant primer result valid Germany rabbit’s feed Vitakraft - + - + biotofu, Edeka - - - + fresh corn leaves Gottmadingen - + - + fresh corn - - - ? bio-corn Seeberger - - - + polenta Kattus - - - + tortilla chips, Delvita - + - + soy sprouts, bamboo garden - - - + tobacco, Lucky Strike - - - + Results of the investigation of GMO in food

  7. USA GMO primer plant primer results valid America’s choice corn starch - + - + America’s choice muffin mix - + - + All natural corn starch - - - + Agro Pure corn starch - - - + Quaker, corn meal - + - + Aunt Jamina corn meal mix - + - + Jiffy corn muffin mix - + - + fresh corn leaves - + - + fresh corn - + - + negative c. - + - + positve c. + + + + Results of the investigation of GMO in food

  8. Conclusion

  9. Suggestions and next steps • DNA is digested without sample buffer , even when it‘s boiled : Buffer contains complexing substances for Ca2+ and Mg2+ • Gels with 3 % (w/v) agarose : tends to keep air bubbles • High concentration of agarose and buffer : the gel swells • - Problem: the wells will become smaller and smaller : difficult to load - remove comb just before loading • PCR is not influenced by any mistakes • For electrophoresis: put samples + control samples of the same primer together in 1 line • – you can save space! • Two combs fits in one gel – that saves space as well ! • => For statistic purpose : sent us more samples !!! • Thank you for your attention

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