1 / 10

LMD of Sperm from a Cell Mixture

LMD of Sperm from a Cell Mixture. Why is cell separation important?. DNA mixtures are frequently encountered in forensic evidence particularly in sexual assault crimes. Identify individuals involved with a crime

arebecca
Download Presentation

LMD of Sperm from a Cell Mixture

An Image/Link below is provided (as is) to download presentation Download Policy: Content on the Website is provided to you AS IS for your information and personal use and may not be sold / licensed / shared on other websites without getting consent from its author. Content is provided to you AS IS for your information and personal use only. Download presentation by click this link. While downloading, if for some reason you are not able to download a presentation, the publisher may have deleted the file from their server. During download, if you can't get a presentation, the file might be deleted by the publisher.

E N D

Presentation Transcript

  1. LMD of Sperm from a Cell Mixture

  2. Why is cell separation important? • DNA mixtures are frequently encountered in forensic evidence particularly in sexual assault crimes. • Identify individuals involved with a crime • Genotype mixture interpretation and statistical analysis can be challenging and time consuming • Separation of different cell types can provide cell fractions from individuals involved in a crime.

  3. Separation Methods • Preferential Lysis (differential extraction) • Flow Cytometry • Microchip separation • Membrane Filtration • Magnetic Antibodies • Y- chromosome (non-physical separation) • Laser Microdissection

  4. LMD Technology Microscope Objective Slide  Specimen Tube 

  5. Sperm & Epithelial Cell Mixture • Technical Issues of Sperm ID under LMD using brightfield conditions • PEN film architecture • Stain is necessary • It helps to adjust focal plane • May be resolved by new EP “Director Slides” • High density of epithelial cells • May need to dilute pellet over larger area • No cover slip • Looking through 1mm slide • Set correction collar objective

  6. Identification of sperm cells Leica AS LMD instrument (older version of software shown)

  7. After Laser Microdissection Sperm cuttings in tube

  8. Epithelial Cell Recovery Epithelial cell in collection tube

  9. Current LMD Collection Software

  10. Warning! Laser in Use

More Related