Ch. 8 Membrane Structure & Function

1. Ch. 8 Membrane Structure & Function

2. Membrane Structure and Function • The plasma membrane controls traffic into and out of the cell it surrounds • It is selectively permeable – allows some substances to cross it more easily than others • Phospholipids make up most plasma membranes

3. Fluid Mosaic Model • The membrane is a fluid structure with various proteins embedded in or attached to a double layer of phospholipids

4. Fluid Mosaic Model (a) Lipids move laterally in the membrane, but flip-flopping across the membrane is rare (b) Unsaturated hydrocarbon tails have kinks that keep the molecules from packing together, enhancing fluidity (c) Cholesterol reduces membrane fluidity at moderate temps. by reducing phospholipid movement, but at low temps. it hinders solidification by disrupting the regular packing of phospholipids

5. Fluid Mosaic Model • The main structure to the plasma membrane is the phospholipid bilayer, but proteins determine the membrane’s specific function • Integral proteins – transmembrane proteins with hydrophobic regions that completely span the hydrophobic interior of the membrane • Peripheral proteins – Protein appendages loosely bound to the surface of the membrane and not embedded in the lipid bylayer

6. Fluid Mosaic Model

7. Some Functions of Membrane Proteins

8. Some Functions of Membrane Proteins Videos

9. 6 Functions of Membrane Proteins • Anchoring proteins (stabilizers): • attach to inside or outside structures • Recognition proteins (identifiers): • label cells normal or abnormal • Enzymes: • catalyze reactions • Receptor proteins: • bind and respond to ligands (ions, hormones) • Carrier proteins: • transport specific solutes through membrane • Channels: • regulate water flow and solutes through membrane

10. Transport Proteins • A transmembrane protein that helps a certain substance to cross the membrane • The transport protein is specific for the substances it moves across the membrane • Some have hydrophilic channels that substances move through, others hold onto to the “passengers” and physically move them across the membrane • There are two modes of membrane movement: passive transport and active transport

11. The structure of a transmembrane protein

12. Traffic Across Membranes • Substances that move across the membrane do so at different rates

13. Passive Transport • Passive transport does not require energy from the cell • Diffusion – the tendency for molecules of any substance to spread out into the available space • Substances always diffuse down its concentration gradient – from an area of greater concentration to an area of lesser concentration • Diffusion is spontaneous because it decreases free energy

14. Diffusion

15. Figure 8.10 The diffusion of solutes across membranes

16. Osmosis • Osmosis is the passive transport (diffusion) of water across a selectively permeable membrane • Hypertonic– the solution with a higher concentration of solutes • Hypotonic – the solution with a lower concentration of solutes • Isotonic – solutions of equal solute concentration

17. Water Balance Within Cells • Osmoregulation is the control of water balance within living cells • Animal cells do not have cell walls • If in a hypotonic solution, the cell takes on water quickly and can burst (lyse) • If in a hypertonic solution, the cell loses water and shrivels usually leading to death • Cells usually live in an isotonic environment

18. Water Balance Within Cells

19. Water Balance Within Cells • Paramecium have a contractile vacuole to get rid of excess water

20. Figure 8.13 The contractile vacuole of Paramecium: an evolutionary adaptation for osmoregulation

21. Water Balance Within Cells • Plant, prokaryote, fungi, and some protist cells dohave cell walls • If in a hypotonicsolution, the cell takes on water, but the cell wall prevents bursting • Turgor pressure keeps plants upright • If in a hypertonic solution, the cell loses water and pulls away from the cell wall • Plasmolysis usually kills the cell • In an isotonic environment, water does not enter the cell • Causes wilting (cells become flaccid)

22. Water Balance Within Cells

23. Water Balance Within Cells

24. Facilitated Diffusion • Use of transport proteins to move substances across the plasma membrane This transport protein alternates between two shapes to move substances through A channel protein allows substance to pass through

25. Some Functions of Membrane Proteins Video Transport Proteins

26. Active Transport • The cell must expend energy (ATP) to move substance against the concentration gradient (from low concentration to high concentration)

27. Figure 8.15 The sodium-potassium pump: a specific case of active transport

28. Comparison of Transport Types

29. Exocytosis • Secretion of macromolecules by the fusion of vesicles with the plasma membrane • A transport vesicle that has budded from the Golgi apparatus moves to the plasma membrane • When it reaches the membrane, the vesicle fuses with it and releases its contents to the outside of the cell

30. Exocytosis

31. Endocytosis • The cell takes in macromolecules and particulate matter by forming new vesicles from the plasma membrane • Three types • Phagocytosis – the cell engulfs large particles by forming a vacuole from the cell membrane; the vacuole fuses with a lysosome to be digested • Pinocytosis – droplets of fluid are taken into the cell

32. Endocytosis

33. Receptor-Mediated Endocytosis • The movement of specific molecules into a cell by the inward budding of vesicles containing proteins with receptor sites specific to the molecules being taken in • This enables a cell to acquire bulk quantities of specific substances.

34. Receptor-Mediated Endocytosis

35. Figure 8.19 The three types of endocytosis in animal cells