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Denaturation of DNA Causes Hyperchromism

Denaturation of DNA Causes Hyperchromism. 1.3 1.2 1.1 1.0. 50 70 90. Relative absorbance (260 nm). T m. Adapted from Voet et al (1999) Fundamental of Biochemistry p.739. Temperature (C). G+C Content Is Proportional to Tm. 1.4 1.2 1.0.

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Denaturation of DNA Causes Hyperchromism

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  1. Denaturation of DNA Causes Hyperchromism 1.3 1.2 1.1 1.0 50 70 90 Relative absorbance (260 nm) Tm Adapted from Voet et al (1999) Fundamental of Biochemistry p.739 Temperature (C)

  2. G+C Content Is Proportional to Tm 1.4 1.2 1.0 70 80 90 100 Pneumococcus (38%) G+C E. coli (52%) S. Marcescens (58%) Relative absorbance (260 nm) M. Phlei (66%) Adapted from Garrett & Grisham (1999) Biochemistry (2e) p.372 Temperature (C)

  3. Cation Neutralizes Phosphate and Elevates Tm 3’ 5’ 100 80 60 40 20 0 60 70 80 90 100 110 5’ 3’ 0.01 M phosphate 0.001 M EDTA G + C (%) 0.15 M NaCl 0.015 M Na citrate Tm (C) Adapted from Garrett & Grisham (1999) Biochemistry (2e) p.372

  4. G+C Content Is Proportional to DNA Density 80 60 40 20 M. phlei Serratia E. coli Calf thymus Salmon sperm Pneumococcus 1.69 1.70 1.71 1.72 1.73 1.74 G + C (%) Adapted from Garrett & Grisham (1999) Biochemistry (2e) p.375 Density (r)

  5. Renaturation Time Is Proportional to Complexity 0 0.5 1.0 10-6 10-5 10-4 10-3 10-2 10-1 1 10 100 1K 10K Time for renaturaton (Longer) Genome size 1 10 102 103 104 105 106 107 108 109 1010 E. coli Poly U/ Poly A MS2 T4 Calf Mouse satellite Fraction reassociated c0t (mole/L •sec) Adapted from Garrett & Grisham (1999) Biochemistry (2e) p.374

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