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Jaroslav NOVÁK , Richard TYKVA, Věra VLASÁKOVÁ, Tomáš RUML

APPLICATION OF LIQUID SCINTILLATION SPECTROMETRY TO THE ANALYSIS OF DEGRADATION AND TOXICITY OF PESTICIDES. Jaroslav NOVÁK , Richard TYKVA, Věra VLASÁKOVÁ, Tomáš RUML. Equipment arragnement. HPLC- isolation. labeled. compound. extraction. tissue culture. extraction. Used diastereoisomers.

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Jaroslav NOVÁK , Richard TYKVA, Věra VLASÁKOVÁ, Tomáš RUML

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  1. APPLICATION OF LIQUID SCINTILLATION SPECTROMETRY TO THE ANALYSIS OF DEGRADATION AND TOXICITY OF PESTICIDES Jaroslav NOVÁK, Richard TYKVA, Věra VLASÁKOVÁ, Tomáš RUML

  2. Equipment arragnement

  3. HPLC- isolation labeled compound extraction tissue culture extraction

  4. Used diastereoisomers

  5. Radiochromatogram of the biodegradation products of cis- isomer

  6. Biodegradation of diastereoisomers

  7. Biodegradation products of diaseteroisomers

  8. Toxicity of diastereoisomers and their biodegradation products 120 100 80 Rel. luminiscence [%] 60 40 20 0 0 10 20 30 40 50 60 70 80 90 100 Concentration [µg/mL] Ecotoxicity expressed as a relative luminescence for different concentrations ¨ b+c of the analyzed compounds: x cis - isomer; trans - isomer;  fractions; a fraction.  o Cultivation: 72 hours, 37 C, 5% (v/v) CO 2

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