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Molecular Immunology at IGM Who? Where? When? Why and What for?

Molecular Immunology at IGM Who? Where? When? Why and What for?. Gudrun Weiss. 5. floor at IGM at the end of “højhuset”. Where?. When?. January 2009. DTU KU LIFE. Continous Non Stop Process. Molecular Immunology Group. Transformation Process. 1 Associate Professor 1 Post Doc

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Molecular Immunology at IGM Who? Where? When? Why and What for?

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  1. Molecular Immunology at IGM Who? Where? When? Why and What for? Gudrun Weiss

  2. 5. floor at IGM at the end of “højhuset” Where? When? January 2009 DTU KU LIFE

  3. Continous Non Stop Process Molecular Immunology Group Transformation Process 1 Associate Professor 1 Post Doc 2 PhD Students 2 Lab Technicians 1 Lab Tech Trainee Work Flow Hanne Frøkiær

  4. Stine Louise Hanne Kristina Molecular Immunology Group Who?

  5. The Real Engine of the Lab Anni Shadi Marianne

  6. Immune stimulatory effects? PhD Student Kristina Galathea Expedition Marine Bacteria Collaboration with DTU Aqua

  7. Kristina's Winter 2010-11

  8. 5’ 3’ miRNA (micro RNA) Small non coding RNA -- ug c ug a --------- u ua aau uag gaa cauuucugcaca aac a au uua auc cuu guaaaggcgugu uug a gg gu c gucgaccguag u Regulation of Biological Processes Associate Professor Stine DNA RNA Protein

  9. PhD Student Louise Uptake of bacteria in immune cells Endocytosis

  10. Research Topic: Immunological Recognition and Response to Microorganisms • How are Microorganisms recognized? • Which Cellsinteract with Microbes? • What determines the Response to Microbes? • How is a Microbe recognized as a Pathogen? Special Focus:Gastro-Intestinal Tract

  11. Why and What for?

  12. The Gastrointestinal Tract • One of the key organs in the body • Largesurface area: 150 - 200 m2 (skin: only 2 m2) • Surface small intestine: Size of a Tennis Court

  13. The Human Microbiota Human Gastro-Intestinal Tract ~ 1x1014 bacteria, several thousand different species •10 x more bacteria cells than human cells •100 x more bacteria genes than human genes

  14. Probiotics • - “Pro Bio” – “For life” • - Gram positive Lactic Acid Bacteria • - Lactobacilli • - Bifidobacteria • - Natural inhabitants of the intestine, “Friendly Bacteria” • Beneficial Effects: Infectious diarrhea (especially rotavirus) • Inflammatory bowel disease, Lactose intolerance, Allergies

  15. Intestinal Tract

  16. Excretion of immunstimulatory factors Immune response in the body Dendritic Cells

  17. Genes: Cells • Real-Time PCR • Microarray Proteins: Supernatant • ELISA Stimulation Assay + Growth factor GM-CSF 8 days: Immature Dendritic Cells

  18. How do Probiotic Bacteria • stimulate the Immunsystem? • Lactobacillus acidophilus • Bifidobacterium bifidum Up/Downregulation of Genes in Dendritic cells Elucidation of Pathways

  19. Pro-inflammatory Lactobacilli Bifidobacteria Anti-inflammatory

  20. Mechanisms? MICROARRAY Inhibition of pro-inflammatory interleukin IL-12 - Lactobacillus Acidophilus - Bifidobacterium Bifidum ELISA Proteins

  21. L. acidophilus NCFM Response to Virus Microarray Analysis Screening: >34.000 Genes

  22. Activation of the Viral Immune System Expression of the key regulator: Interferon β (IFN- β) Protection against influenza?!?

  23. ssRNA DNA dsRNA ? JNK pathway Mechanisms – Use of Knock Out Mice Lactobacillus acidophilus Virus + Pathogenic bacteria

  24. My Winter 2010-11

  25. General Atmosphere in the Lab?

  26. Paper rejected 

  27. Paper accepted 

  28. Have a nice Weekend!!! Any Questions???

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