1 / 25

Diagnostic Laboratory Procedures

Diagnostic Laboratory Procedures. Agriculture, Food, and Natural Resource Standards Addressed. AS.07.01. Design programs to prevent animal diseases, parasites and other disorders and ensure animal welfare.

arnoldmark
Download Presentation

Diagnostic Laboratory Procedures

An Image/Link below is provided (as is) to download presentation Download Policy: Content on the Website is provided to you AS IS for your information and personal use and may not be sold / licensed / shared on other websites without getting consent from its author. Content is provided to you AS IS for your information and personal use only. Download presentation by click this link. While downloading, if for some reason you are not able to download a presentation, the publisher may have deleted the file from their server. During download, if you can't get a presentation, the file might be deleted by the publisher.

E N D

Presentation Transcript

  1. Diagnostic Laboratory Procedures

  2. Agriculture, Food, and Natural Resource Standards Addressed • AS.07.01. Design programs to prevent animal diseases, parasites and other disorders and ensure animal welfare. • AS.07.01.01.b. Describe and demonstrate the proper use and function of specific tools and technology related to animal health management.

  3. Microscope Use • Objective lenses 4x, 10x, and 40x • Oil-immersion is used occasionally • A mechanical stage is necessary to view slides thoroughly

  4. Microscope Use • Viewing area = field

  5. Microscope Use • To scan a slide use 4x to focus, then move to 10x • Scan back and forth overlapping each field

  6. Microscope Use Scanning pattern

  7. Microscope Use • Debris can be closely examined under high power

  8. Sample Collection • The animal owner will most often collect the stool sample.

  9. Sample Collection • The sample should be fresh since some parasite eggs become unrecognizable as the feces ages.

  10. Sample Collection • If a fresh sample cannot be obtained, one can be refrigerated, but for no longer than 24 hours.

  11. Methods • Gross exam - used to observe outward characteristics of the sample.

  12. Methods • Gross exam - Several abnormalities can be seen with the naked eye.

  13. Methods • Gross exam - observe and record • color and consistency • blood and mucus • age of sample • presence of adult parasites

  14. Methods 2. Direct smear - the fastest and simplest method of diagnosing parasitism.

  15. Methods 2. Direct smear - A small amount of feces is mixed with water and applied directly to a slide.

  16. Methods 2. Direct smear - The main disadvantage of this technique is that a small sample may not contain any parasite eggs.

  17. Methods 2. Direct smear - This method also leaves debris on the slide.

  18. Methods 3. Floatation - the most commonly used procedure for diagnosing parasitism.

  19. Methods 3. Floatation - techniques using this method: - simple floatation - use of a centrifuge - Fecalyzer

  20. Methods 3. Floatation Fecalyzer - uses a solution (zinc sulfate or sodium nitrate) that has a specific gravity greater than the specific gravityof most parasite eggs

  21. Methods 4. Sedimentation - is commonly used for eggs that have a high specific gravity

  22. Methods 4. Sedimentation - The high specific gravity of these eggs makes it difficult to use floatation techniques without distorting them.

  23. Methods 4. Sedimentation - Sedimentation allows eggs to sink to the bottom.

  24. Debris • may be easily confused with parasite eggs.

  25. Common Debris: • fat • epithelial cells • pollen grains • hair • plant material • air bubbles

More Related