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BSCI 338K - Lecture 3 Developmental genetics of sex determination

BSCI 338K - Lecture 3 Developmental genetics of sex determination. Thomas D. Kocher Department of Biology. Migration of Primordial Germ Cell (PGCs). Functional gene network analysis of testis development genes.

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BSCI 338K - Lecture 3 Developmental genetics of sex determination

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  1. BSCI 338K - Lecture 3Developmental genetics of sex determination Thomas D. Kocher Department of Biology

  2. Migration of Primordial Germ Cell (PGCs)

  3. Functional gene network analysis of testis development genes 109 genes with 1.5-fold change in expression from e13-e16 in the mouse gonad. Cell processes involved in testis development were determined based on the number of arrows connected to each box (connectivity). (Clement et al. 2007)

  4. Genetic interactions

  5. Developmental pathway

  6. Central switch? (DiNapoli and Capel, 2007)

  7. Vertebrate sex determination SRY Ezaz et al. 2006

  8. Tilapia (Genomar)

  9. Sex-linked markers in Oreochromis niloticus: LG1 Ph.D thesis of Bo-Young Lee (Lee 2003)

  10. X/X X/Y LG 1 W/Z LG 3 Z/Z Epistasis in O. aureus (Lee 2004)

  11. LG1 and LG3 are distinct chromosomes (Cnaani 2007)

  12. Sex-linked markers in tilapia

  13. Sex determination in Malawi cichlids M.S. thesis of Jennifer Ser • Survey of 19 species in five genera • 94 families (2066 offspring) • Extensive variation in sex ratios • Genotyped for microsatellite markers • Focused on LG 5 and 7

  14. Metriaclima phaeos - XY on LG7

  15. Metriaclima phaeos - WZ on LG5

  16. M. fainzilberi - WZ and XY

  17. Grand summary…

  18. Hidden complexity of genetic interactions LG5 WZ (OB species) LG? WZ M. pyrsonotus LG7 XY (widespread) LG? XY M. kompakt LG? M. callainos LG? WZ M. lombardoi

  19. Metriaclima lombardoi 44 chromosomes + B chromosome (some females) Duplication in largest pair (some males) Irani Ferreira

  20. Sex determiners in African cichlidsTilapia Malawi

  21. Candidate gene on LG1

  22. Ijiri et al. 2006

  23. Quantitative PCR of mRNA Tilapia gonads 0-70 days Ijiri et al. 2006

  24. Project Opportunities: Sex Determination • Fine map the LG1 XY locus in Nile tilapia • Fine map the LG7 XY locus in Malawi cichlids • Map new sex determiners in Malawi cichlids • Map sex determiners in Lake Victoria cichlids • Map sex determining locus in Astatotilapia burtoni • Annotate sex determining regions of other fishes onto cichlid genome sequence • Characterize expression of candidate genes during gonad development

  25. Proposal Process • Proposal format • Cover page • Abstract (300 words) • Proposal Body (10 pp total) • Background (2-3 pp) • Preliminary data (1-2 pp) • Proposed research (5-6 pp) • Expected Results and timeline (1 pp) • References • Budget • Proposals due **** Feb 17, 2011 **** • Peer reviews due Feb 22, 2011 • Panel meeting Feb 24, 2011 • Project start date March 1, 2011

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