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Epithelial Tissue Lab Exercise: Review of Different Types of Epithelium

This lab exercise focuses on reviewing and identifying different types of epithelial tissues, including simple cuboidal, simple squamous, simple columnar, pseudostratified ciliated columnar, transitional, stratified squamous, stratified columnar, and stratified cuboidal. Follow the instructions in the lab manual and draw at least one example of each type of tissue.

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Epithelial Tissue Lab Exercise: Review of Different Types of Epithelium

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  1. Lab Exercise #6a Part 1 - Epithelial Tissues

  2. Let’s review….

  3. Simple cuboidal epithelium • lining a tubule (longitudinal cut). Some of the cell boundaries between "blocks" or "cubes" here are quite distinct.

  4. Simple Cuboidal • Covers surface of ovary, lines kidney tubules and small ducts of glands (thyroid and pancreas)

  5. Tubule - cross sectionsimple cuboidal

  6. Another example - kidney

  7. Type?

  8. Simple squamous epithelium • High power view of endothelial cells lining a small blood vessel cut in cross-section. (You see just the nuclei - the cytoplasm between them is extremely flat.) Endothelium = the simple squamous epithelium lining blood vessels.

  9. Simple squamous epithelium

  10. Kidney

  11. Superficial view (squamous)

  12. Type?

  13. Simple columnar • Another example – intestine, with goblet

  14. Intestinal lining

  15. Yet another

  16. Type?

  17. Pseudostratified ciliated columnar epithelium Trachea. Nuclei are at different levels. All cells touch the basement membrane, but only the taller cells reach the lumen. The cilia are longer and less regular than the microvilli of a striated border.

  18. Pseudostratified Columnar Epithelium • Look like multiple layers • Trachea Figure 4–5b

  19. Another example

  20. Cilia – surface view

  21. Type?

  22. Bladder Low power

  23. Transitional Epithelium • Tolerates stretching • Appearance changes (stratified to simple) • Urinary bladder Figure 4–4c

  24. Type?

  25. Stratified Squamous Epithelium Figure 4–3b

  26. Example

  27. Example

  28. Type?

  29. Type?

  30. Stratified Cuboidal Epithelium • Sweat gland ducts Figure 4–4b

  31. Type?

  32. Stratified Columnar Epithelium • Rare • Salivary gland duct Figure 4–5c

  33. Note • It is the shape of the most superficial layer that determines what type of stratified epithelium it is.

  34. Today’s procedure • Set up microscope • Get a slide (or up to 3 or 4) • Follow instructions in Lab Manual • Hints • Start on low power and locate epithelium (this is the bulk of the work) • Go to 10x, then 40x • Draw tissue • Return slide to box you got it from.

  35. Today’s lab • Examine and draw at least one of each type of epithelial tissue • Activity 1: • Simple squamous • Simple cuboidal • Simple columnar • Psuedostratified ciliated columnar • Transitional • Stratified squamous • Stratified columnar • Stratified cuboidal (?)

  36. To turn in: • 8 drawings on page I provide • Review sheet

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